Invitrogen

T-bet Monoclonal Antibody (eBio4B10 (4B10)), PE-Cyanine5, eBioscience™

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T-bet Antibody (15-5825-82) in Flow

Normal human peripheral blood cells were stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with CD8a Monoclonal Antibody, eFluor 450 (Product # 48-0087-42) and 0.125 µg of Mouse IgG1 kappa Isotype Control, PE-Cyanine5 (Product # 15-4714-81) (left) or 0.125 µg of T-bet Monoclonal Antibody, PE-Cyanine5 (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

15-5825-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

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产品规格

种属反应

Human, Mouse, Rhesus monkey

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBio4B10 (4B10)

偶联物

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

激发/发射光谱

568/666 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2815071

产品详细信息

Description: The eBio4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN gamma, and T-Bet also regulates the expression of IFN gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN gamma, T-Bet is also known to regulate the expression of IL-12R beta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.

Applications Reported: This eBio4B10 (4B10) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBio4B10 (4B10) antibody has been tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at www.thermofisher.com/flowprotocols . This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer ((Product # 00-8222-49)) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution ((Product # 00-5333-57)) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

靶标信息

T-bet, also known as TBX21, is a T-cell specific transcription factor containing a single T-box DNA-binding domain. It controls the expression of the TH1 cytokine, interferon-gamma. Both Th1 and Th17 cells are crucial in immune regulation and autoimmune disease development. T-bet initiates Th1 lineage development from naive Th precursor cells and directs T cell differentiation to Th1 versus Th17. T-bet is cooperating with Stat4 (signal tranducer and activator of transcription 4) in programming chromatin architecture for Th1 gene expression. Genetic variations in T-bet are associated with susceptibility to aspirin-induced asthma and nasal polyps.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

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Nature communications

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Thu Oct 12 00:00:00 EDT 2023

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International journal of molecular sciences

Chan L,Mehrani Y,Minott JA,Bridle BW,Karimi K

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Thu Jan 26 00:00:00 EST 2023

GM-CSF-activated human dendritic cells promote type 1 T follicular helper cell polarization in a CD40-dependent manner.

Journal of cell science

Korniotis S,Saichi M,Trichot C,Hoffmann C,Amblard E,Viguier A,Grondin S,Noel F,Mattoo H,Soumelis V

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Tue Nov 01 00:00:00 EDT 2022

Agr2-associated ER stress promotes adherent-invasive E. coli dysbiosis and triggers CD103⁺ dendritic cell IL-23-dependent ileocolitis.

Cell reports

Viladomiu M,Khounlotham M,Dogan B,Lima SF,Elsaadi A,Cardakli E,Castellanos JG,Ng C,Herzog J,Schoenborn AA,Ellermann M,Liu B,Zhang S,Gulati AS,Sartor RB,Simpson KW,Lipkin SM,Longman RS

Published figure using T-bet monoclonal antibody (Product # 15-5825-82) in Flow Cytometry

Tue Nov 15 00:00:00 EST 2022

Impact of secondary TCR engagement on the heterogeneity of pathogen-specific CD8+ T cell response during acute and chronic toxoplasmosis.

PLoS pathogens

Shallberg LA,Phan AT,Christian DA,Perry JA,Haskins BE,Beiting DP,Harris TH,Koshy AA,Hunter CA

15-5825-82 was used in flow cytometry to highlight that recent TCR activation contributes to the phenotypic heterogeneity of the CD8+ T cell response but suggest that this process has a limited impact on memory populations at acute and chronic stages of infection.

Wed Jun 01 00:00:00 EDT 2022

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Cell reports

VanDyke D,Iglesias M,Tomala J,Young A,Smith J,Perry JA,Gebara E,Cross AR,Cheung LS,Dykema AG,Orcutt-Jahns BT,Henclová T,Golias J,Balolong J,Tomasovic LM,Funda D,Meyer AS,Pardoll DM,Hester J,Issa F,Hunter CA,Anderson MS,Bluestone JA,Raimondi G,Spangler JB

15-5825-82 was used in Flow cytometry/Cell sorting to provide a roadmap for IC design and establish a Treg-biased immunotherapy that could be clinically translated for autoimmune disease treatment.

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Cellular & molecular immunology

Liu Y,Bockermann R,Hadi M,Safari I,Carrion B,Kveiborg M,Issazadeh-Navikas S

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Imbalance between T helper 1 and regulatory T cells plays a detrimental role in experimental Parkinson's disease in mice.

The Journal of international medical research

Li W,Luo Y,Xu H,Ma Q,Yao Q

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Thu Apr 01 00:00:00 EDT 2021

Francisella tularensis induces Th1 like MAIT cells conferring protection against systemic and local infection.

Nature communications

Zhao Z,Wang H,Shi M,Zhu T,Pediongco T,Lim XY,Meehan BS,Nelson AG,Fairlie DP,Mak JYW,Eckle SBG,de Lima Moreira M,Tumpach C,Bramhall M,Williams CG,Lee HJ,Haque A,Evrard M,Rossjohn J,McCluskey J,Corbett AJ,Chen Z

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Fri Jul 16 00:00:00 EDT 2021

Heterologous arenavirus vector prime-boost overrules self-tolerance for efficient tumor-specific CD8 T cell attack.

Cell reports. Medicine

Bonilla WV,Kirchhammer N,Marx AF,Kallert SM,Krzyzaniak MA,Lu M,Darbre S,Schmidt S,Raguz J,Berka U,Vincenti I,Pauzuolis M,Kerber R,Hoepner S,Günther S,Magnus C,Merkler D,Orlinger KK,Zippelius A,Pinschewer DD

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Tue Mar 16 00:00:00 EDT 2021

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Cell reports

Budhu S,Giese R,Gupta A,Fitzgerald K,Zappasodi R,Schad S,Hirschhorn D,Campesato LF,De Henau O,Gigoux M,Liu C,Mazo G,Deng L,Barker CA,Wolchok JD,Merghoub T

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STAR protocols

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Cell reports. Medicine

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Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

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Mon May 31 00:00:00 EDT 2021

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Science advances

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Cell reports

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Single-Cell Profiling Defines Transcriptomic Signatures Specific to Tumor-Reactive versus Virus-Responsive CD4⁺ T Cells.

Cell reports

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生物信息学

蛋白别名: HGNC:11599; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; Transcription factor TBLYM

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基因别名: T-bet; T-PET; TBET; TBLYM; TBT1; TBX21

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UniProt ID: (Human) Q9UL17, (Mouse) Q9JKD8

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Entrez Gene ID: (Rhesus monkey) 694044, (Human) 30009, (Mouse) 57765

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功能

transcription factor activity, sequence-specific DNA binding transcription regulatory region DNA binding DNA binding protein binding Rel homology transcription factor

参与通路

transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development response to virus T cell differentiation positive regulation of transcription, DNA-templated positive regulation of isotype switching to IgG isotypes cellular response to organic substance lymphocyte migration regulation of immune response