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TCL1A Antibody (MA5-50732) in ICC/IF
Immunocytochemistry analysis of TCL1A in PC-3 cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with TCL1A recombinant monoclonal antibody (Product # MA5-50732) with a dilution of 1:50 in 2% negative goat serum overnight at 4 &(8)451;. Followed by secondary antibody Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. PBS instead of the pri... View More
产品信息
MA5-50732
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
PD00-94
抗原
Synthetic peptide aa 1-114/114.
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_3092880
产品详细信息
Positive control: Daudi cell lysates, human tonsil tissue, human appendix tissue, PC-3, Daudi.
Predicted band size: 13 kDa
Subcellular Location: Cytoplasm, Nucleus, Microsome, Endoplasmic reticulum.
靶标信息
Overexpression of the TCL1 gene in humans has been implicated in the development of mature T cell leukemia, in which chromosomal rearrangements bring the TCL1 gene in close proximity to the T-cell antigen receptor (TCR)-alpha or TCR-beta regulatory elements (summarized by Virgilio et al., 1998). In normal T cells TCL1 is expressed in CD4-/CD8- cells, but not in cells at later stages of differentiation. TCL1 functions as a coactivator of the cell survival kinase AKT (Laine et al., 2000).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Oncogene TCL-1; Oncogene TCL1; Protein p14 TCL1; T-cell leukemia/lymphoma protein 1A; T-cell lymphoma-1; T-cell lymphoma-1A
基因别名: TCL1; TCL1A
UniProt ID: (Human) P56279
Entrez Gene ID: (Human) 8115
