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TCP-1 beta Antibody (PA5-78952) in ICC/IF
Immunocytochemistry analysis of CCT2 using anti-CCT2 antibody (Product # PA5-78952) . CCT2 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-CCT2 antibody (Product # PA5-78952) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG... View More
产品信息
PA5-78952
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human CCT2 recombinant protein (Position: E414-C535).
偶联物
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.01mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2746068
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human A549 whole cell, human RT4 whole cell, human CACO-2 whole cell, human 293T whole cell, human MCF-7 whole cell, human HEL whole cell, human Raji whole cell, human SW620 whole cell, rat brain tissue, rat spleen tissue, rat C6 whole cell, rat PC-12 whole cell, mouse brain tissue, mouse spleen tissue, mouse NIH/3T3 whole cell, mouse RAW2647 whole cell. IHC: rat gaster tissue, human testis tissue, mouse gaster tissue. ICC/IF: U20S cell, U2OS cell. Flow: U251 cell.
靶标信息
TCP-1 beta is a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). The CCT complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of the TCP-1 beta gene have been observed but not thoroughly characterized. A disease associated with TCP-1 beta protein dysfunction include epididymo-orchitis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AI528772; c-cpn; CCT; CCT-beta; Cct1; Ccta; Chaperonin containing T-complex polypeptide 1 subunit 2; chaperonin containing t-complex polypeptide 1, beta subunit; chaperonin containing t-complex polypeptide 1, subunit 2; chaperonin containing TCP1, subunit 2 (beta); chaperonin subunit 2 (beta); epididymis secretory sperm binding protein Li 100n; p63; T-complex protein 1 subunit beta; T-complex protein 1, beta subunit; Tcp-1; TCP-1-beta; Tp63; TRic
基因别名: 99D8.1; CCT-beta; CCT2; CCTB; HEL-S-100n; PRO1633; TCP-1-beta
UniProt ID: (Human) P78371, (Mouse) P80314, (Rat) Q5XIM9
Entrez Gene ID: (Human) 10576, (Mouse) 12461, (Rat) 299809
