Invitrogen

TCTN3 Polyclonal Antibody

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TCTN3 Antibody (PA5-87479) in WB

Western blot analysis of TCTN3 in extracts of various cell lines. Samples were incubated with TCTN3 Polyclonal antibody (Product # PA5-87479) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

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产品信息

PA5-87479

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 200-475 of human TCTN3 (NP_0564464)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.85 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2804185

产品详细信息

Immunogen sequence: QTQSPPSFYR AGDPILTYFP KWSVISLLRQ PAGVGAGGLC AESNPAGFLE SKSTTCTRFF KNLASSCTLD SALNAASYYN FTVLKVPRSM TDPQNMEFQV PVILTSQANA PLLAGNTCQN VVSQVTYEIE TNGTFGIQKV SVSLGQTNLT VEPGASLQQH FILRFRAFQQ STAASLTSPR SGNPGYIVGK PLLALTDDIS YSMTLLQSQG NGSCSVKRHE VQFGVNAISG CKLRLKKADC SHLQQEIYQT LHGRPRPEYV AIFGNADPAQ KGGWTR; Positive Samples: Mouse craniofacial, Mouse lung, Mouse brain; Cellular Location: Membrane, Single-pass type I membrane protein

靶标信息

TCTN3 is a member of the Tectonic protein family, a group of evolutionarily conserved secreted and transmembrane proteins that regulate the Hedgehog (Hh)-mediated patterning of the neural tube. While the expression pattern and function of TCTN3 is not known, it is 58% similar to TCTN1. TCTN1 is expressed during embryonic development in regions that participate in Hh signaling, beginning in the gastrulation stages in the ventral node. Mice expressing mutant TCTN1 die between E13.5 and E16.5 and display holoprosencephaly, a defect associated with reduced Hh signaling, indicating the role of TCTN1 as an Hh activator. At later stages in development, TCTN1 is thought to also act as a repressor on the Hh pathway in the anterior and posterior neural tube.

仅用于科研。不用于诊断过程。未经明确授权不得转售。