OriGene

TDG Monoclonal Antibody (OTI3C11), TrueMAB™

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

查看其他16个TDG抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (15)
高级验证 (1)

TDG Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TDG Antibody (CF808610) in IHC (P)

Immunohistochemical staining of paraffin-embedded human lymphoma tissue using anti-TDG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808610)(1:150).

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TDG Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in TDG knockout (KO) Hela cell line using Anti-TDG monoclonal antibody (Product # CF808610). {KO}

产品信息

CF808610

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫组化（石蜡） (IHC (P))

1:150

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

OTI3C11

抗原

Human recombinant protein fragment corresponding to amino acids 160-253 of human TDG produced in E.coli.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 8% trehalose

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

靶标信息

The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mis paired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.

仅用于科研。不用于诊断过程。未经明确授权不得转售。