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TEV Cleavage Site Antibody (PA1-119) in WB
Western blot analysis of TEV cleavage site was performed by loading the indicated amounts of TEV control protein and 5 µL of Lane Marker Reducing Sample Buffer (Product # 39000) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a TEV cleavage site polyclonal antibody (Product # PA1-119) at a dilution of 1:500 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-rabbit IgG-HR... View More
产品信息
PA1-119
产品规格
种属反应
Tag
已发表种属
Tag
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Peptide representing the cleavage site recognized by TEV enzyme
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Antigen affinity chromatography
保存液
BBS, pH 8.4
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2539888
产品详细信息
PA1-119 has been successfully used in Western blot applications.
The PA1-119 immunogen is a peptide representing the cleavage site recognized by TEV enzyme.
靶标信息
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tandem affinity purification (TAP) is an affinity purification method for isolation of TAP-tagged proteins together with associated proteins. The protocol involves the fusion of the "TAP tag" (typically a calmodulin binding peptide (CBP), a tobacco etch virus protease (TEV protease) cleavage site and Protein A) to the protein of interest. The TAP technique is useful in analyzing in vivo interactions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Tobacco Etch Virus
