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Invitrogen
TFEB Polyclonal Antibody
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TFEB Antibody (PA1-31552) in ICC/IF
Immunofluorescence analysis of TFEB was performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TFEB Polyclonal Antibody (Product # PA1-31552) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32814) at ... View More
产品信息
PA1-31552
应用
建议稀释比
已发表文章
产品规格
已发表种属
C. elegans,
Dog,
Human,
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Peptide with sequence ASRIGLRMQLMRE, from N Terminus of the protein sequence according to NP_009093
偶联物
形式
Liquid
浓度
0.5 mg/mL
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
Expected to cross react with human due to sequence homology.
Positive control of NIH-3T3 lysate suggested.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
The Transcription factor EB (TFEB) is a member in the basic helix-loop-helix leucine zipper superfamily of transcription factors that is translocated in a subset of renal tumors. Recent studies have shown that lysosomal biogenesis is regulated by TFEB, which is in turn regulated by the mammalian target of rapamycin (mTOR) complex 1. Other evidence suggests that TFEB coordinates the major steps of the autophagic pathway by driving the expression of autophagy and lysosomal genes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
