Invitrogen

TFEB Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4个已发表图片

6 篇参考文献

查看其他19个TFEB抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

TFEB Antibody in Immunohistochemistry (IHC)

图: 1 / 14

TFEB Antibody (PA5-34360) in IHC

Immunohistochemistry of TFEB (NT) in human lung tissue with TFEB Polyclonal Antibody (Product # PA5-34360) at 2.5 µg/mL.

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TFEB Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of TFEB was observed in B-cell lines such as Raji, Daudi and Ramos as compared to T-cell lines such as Jurkat, MOLT-4 and U-937 (DOI: 10.1128/mcb.10.8.4384) using Anti-TFEB Polyclonal Antibody (Product # PA5-34360) in Western Blot. {RE}

产品信息

PA5-34360

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

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免疫组化 (IHC)

2.5 µg/mL

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免疫细胞化学 (ICC/IF)

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免疫沉淀 (IP)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

An 18 amino acid synthetic peptide near the amino terminus of human TFEB

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2551712

产品详细信息

A suggested positive control is A549 cell lysate.

PA5-34360 can be used with blocking peptide PEP-1403.

靶标信息

The Transcription factor EB (TFEB) is a member in the basic helix-loop-helix leucine zipper superfamily of transcription factors that is translocated in a subset of renal tumors. Recent studies have shown that lysosomal biogenesis is regulated by TFEB, which is in turn regulated by the mammalian target of rapamycin (mTOR) complex 1. Other evidence suggests that TFEB coordinates the major steps of the autophagic pathway by driving the expression of autophagy and lysosomal genes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Transcriptional regulation of autophagy-lysosomal function in BRAF-driven melanoma progression and chemoresistance.

Nature communications

Li S,Song Y,Quach C,Guo H,Jang GB,Maazi H,Zhao S,Sands NA,Liu Q,In GK,Peng D,Yuan W,Machida K,Yu M,Akbari O,Hagiya A,Yang Y,Punj V,Tang L,Liang C

PA5-34360 was used in Immunoprecipitation to identify the mechanisms by which BRAFV600E-melanoma controls the transcriptional machinery of the autophagy-lysosomal pathway.

Fri Apr 12 00:00:00 EDT 2019

Transcriptional regulation of autophagy-lysosomal function in BRAF-driven melanoma progression and chemoresistance.

Nature communications

Li S,Song Y,Quach C,Guo H,Jang GB,Maazi H,Zhao S,Sands NA,Liu Q,In GK,Peng D,Yuan W,Machida K,Yu M,Akbari O,Hagiya A,Yang Y,Punj V,Tang L,Liang C

PA5-34360 was used in Immunoprecipitation to identify the mechanisms by which BRAFV600E-melanoma controls the transcriptional machinery of the autophagy-lysosomal pathway.

Fri Apr 12 00:00:00 EDT 2019

Transcriptional regulation of autophagy-lysosomal function in BRAF-driven melanoma progression and chemoresistance.

Nature communications

Li S,Song Y,Quach C,Guo H,Jang GB,Maazi H,Zhao S,Sands NA,Liu Q,In GK,Peng D,Yuan W,Machida K,Yu M,Akbari O,Hagiya A,Yang Y,Punj V,Tang L,Liang C

PA5-34360 was used in Immunoprecipitation to identify the mechanisms by which BRAFV600E-melanoma controls the transcriptional machinery of the autophagy-lysosomal pathway.

Fri Apr 12 00:00:00 EDT 2019

Transcriptional regulation of autophagy-lysosomal function in BRAF-driven melanoma progression and chemoresistance.

Nature communications

Li S,Song Y,Quach C,Guo H,Jang GB,Maazi H,Zhao S,Sands NA,Liu Q,In GK,Peng D,Yuan W,Machida K,Yu M,Akbari O,Hagiya A,Yang Y,Punj V,Tang L,Liang C

PA5-34360 was used in Immunoprecipitation to identify the mechanisms by which BRAFV600E-melanoma controls the transcriptional machinery of the autophagy-lysosomal pathway.

Fri Apr 12 00:00:00 EDT 2019

HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancer.

Autophagy

Kim SY,Kim HJ,Kim HJ,Kim DH,Han JH,Byeon HK,Lee K,Kim CH

PA5-34360 was used in Western Blotting to report that HSPA5 contributes to head and neck cancer (HNC) survival via maintenance of lysosomal activity; however, a nonthermal plasma (NTP, considered as a next-generation cancer therapy)-treated solution (NTS) inhibits HNC progression through HSPA5-dependent alteration of lysosomal activity.