Invitrogen

TGF alpha Polyclonal Antibody, PeproTech®

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TGF alpha Antibody (500-P16-1MG) in WB

Western Blot: To detect Human TGF-alpha by Western Blot analysis TGF alpha Polyclonal Antibody (Product # 500-P16-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human TGF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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产品信息

500-P16-1MG

应用

建议稀释比

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免疫印迹 (WB)

0.1-0.2 µg/mL

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酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

中和实验 (Neu)

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免疫染色 (IS)

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产品规格

种属反应

Human

已发表种属

Hamster, Human

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Human TGF-alpha

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

RRID

AB_2929562

产品详细信息

AA Sequence of recombinant protein: VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TGF-alpha. Anti-Human TGF-alpha-specific antibody was purified by affinity chromatography employing an immobilized Human TGF-alpha matrix.

Sandwich ELISA: To detect Human TGF-alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TGF-alpha (500-P16Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human TGF-alpha.

Western Blot: To detect Human TGF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human TGF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces anchorage-independence transformation in cultured cells. Human, murine and rat TGF-alpha are cross-species reactive. TGFalpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

仅用于科研。不用于诊断过程。未经明确授权不得转售。