Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 1
TIGIT Antibody (414-9501-82) in Flow
BALB/c mouse splenocytes were stained with CD4 Monoclonal Antibody, eFluor 450 (Product # 48-0042-82) and 0.25 µg of Rat IgG2a kappa Isotype Control, Brilliant Violet 480 (Product # 414-4321-81) (left) or 0.25 µg of TIGIT Monoclonal Antibody, Brilliant Violet 480 (right). Cells were then stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with Foxp3 Monoclonal Antibody, APC (Product # 17-5773-82). Viable CD4-positive cells in the lymphocyte gate were used for analysis, as d... View More
产品信息
414-9501-82
产品规格
种属反应
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
GIGD7
抗原
mTIGIT-Fc treated with 4% PFA
偶联物
激发/发射光谱
437/478 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
RRID
AB_3074109
产品详细信息
Description: The GIGD7 monoclonal antibody reacts with mouse T cell immunoreceptor with Ig and ITIM domains (TIGIT, VSTM3, WUCAM), a 26 kDa protein that is a member of the poliovirus receptor (PVR) family. In mice, the expression of TIGIT has been reported in follicular T helper cells, while in humans, the expression of TIGIT has been reported on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ T cells, and CD8+ T cells. TIGIT is not expressed on B cells or naive CD4+ T cells. TIGIT is upregulated on CD4+ T cells following activation. TIGIT can interact with certain members of the PVR and PVR-like families, including CD155, and also mediates the interaction of NK cells and T cells with antigen presenting cells, fibroblasts and endothelial cells that express PVR and PVR-like proteins.
Applications Reported: This GIGD7 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This GIGD7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Brilliant Violet™ 480 (BV480) is a dye that emits at 479 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.
Our internal testing suggests that Brilliant Violet™ 480 (BV480) is not compatible with methanol-based fixation.
Excitation: 440 nm; Emission: 479 nm; Laser: Violet Laser.
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
靶标信息
Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: T-cell immunoreceptor with Ig and ITIM domains; tigit; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3
基因别名: ENSMUSG00000071552; Vstm3
Entrez Gene ID: (Mouse) 100043314
