Invitrogen

TIGIT Monoclonal Antibody (MBSA43), Alexa Fluor™ 488, eBioscience™

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TIGIT Antibody (53-9500-42) in Flow

Normal human peripheral blood cells were stained with CD4 Monoclonal Antibody, PE-Cyanine7 (Product # 25-0048-42) and Mouse IgG1 kappa Isotype Control, Alexa Fluor 488 (Product # 53-4714-80) (left) or TIGIT Monoclonal Antibody, Alexa Fluor 488 (right). Cells in the lymphocyte gate were used for analysis.

TIGIT Antibody in Immunohistochemistry (IHC)

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产品信息

53-9500-42

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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流式细胞分析 (Flow)

5 µL (0.25 µg)/test

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产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

MBSA43

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2866442

产品详细信息

Description: The MBSA43 monoclonal antibody reacts with human T cell immunoreceptor with Ig and ITIM domains (TIGIT), which is also known as VSTM3 or WUCAM. This 26 kDa protein is a member of the poliovirus receptor (PVR) family. The expression of TIGIT has been reported on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ T cells, and CD8+ T cells, but it is not expressed on B cells or naive CD4+ T cells. TIGIT may be upregulated on naive CD4+ T cells upon activation. TIGIT can interact with certain members of the PVR and PVR-like families, including CD155, and also mediates the interaction of NK and T cells with antigen presenting cells, fibroblasts, and endothelial cells that express PVR and PVR-like proteins.

MBSA43 is reported to crossreact with Rhesus macaque.

Applications Reported: This MBSA43 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MBSA43 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

靶标信息

Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

The TIGIT⁺ T regulatory cells subset associates with nosocomial infection and fatal outcome in COVID-19 patients under mechanical ventilation.

Scientific reports

de Lima MHF,Machado CC,Nascimento DC,Silva CMS,Toller-Kawahisa JE,Rodrigues TS,Veras FP,Pontelli MC,Castro IA,Zamboni DS,Filho JA,Cunha TM,Arruda E,da Cunha LD,Oliveira RDR,Cunha FQ,Louzada-Junior P

Published figure using TIGIT monoclonal antibody (Product # 53-9500-42) in Flow Cytometry

Mon Aug 21 00:00:00 EDT 2023

Impaired TIGIT expression on B cells drives circulating follicular helper T cell expansion in multiple sclerosis.

The Journal of clinical investigation

Asashima H,Axisa PP,Pham THG,Longbrake EE,Ruff WE,Lele N,Cohen I,Raddassi K,Sumida TS,Hafler DA

Published figure using TIGIT monoclonal antibody (Product # 53-9500-42) in Flow Cytometry

Mon Oct 17 00:00:00 EDT 2022

Single-cell RNA sequencing reveals cell heterogeneity and transcriptome profile of breast cancer lymph node metastasis.

Oncogenesis

Xu K,Wang R,Xie H,Hu L,Wang C,Xu J,Zhu C,Liu Y,Gao F,Li X,Wang C,Huang J,Zhou W,Zhou G,Shu Y,Guan X

Published figure using TIGIT monoclonal antibody (Product # 53-9500-42) in Immunocytochemistry

Tue Oct 05 00:00:00 EDT 2021

Correlations of the expression of γδ T cells and their co-stimulatory molecules TIGIT, PD-1, ICOS and BTLA with PR and PIBF in the peripheral blood and decidual tissues of women with unexplained recurrent spontaneous abortion.

Clinical and experimental immunology

Liang Q,Tong L,Xiang L,Shen S,Pan C,Liu C,Zhang H

Published figure using TIGIT monoclonal antibody (Product # 53-9500-42) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

TIGIT⁺ TIM-3⁺ NK cells are correlated with NK cell exhaustion and disease progression in patients with hepatitis B virus‑related hepatocellular carcinoma.

Oncoimmunology

Yu L,Liu X,Wang X,Yan F,Wang P,Jiang Y,Du J,Yang Z

Published figure using TIGIT monoclonal antibody (Product # 53-9500-42) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

SARS-CoV-2-specific T cell memory is sustained in COVID-19 convalescent patients for 10 months with successful development of stem cell-like memory T cells.

Nature communications

Jung JH,Rha MS,Sa M,Choi HK,Jeon JH,Seok H,Park DW,Park SH,Jeong HW,Choi WS,Shin EC

Published figure using TIGIT monoclonal antibody (Product # 53-9500-42) in Flow Cytometry

Wed Jun 30 00:00:00 EDT 2021

TIGIT is upregulated by HIV-1 infection and marks a highly functional adaptive and mature subset of natural killer cells.

AIDS (London, England)

Vendrame E,Seiler C,Ranganath T,Zhao NQ,Vergara R,Alary M,Labbé AC,Guédou F,Poudrier J,Holmes S,Roger M,Blish CA

Published figure using TIGIT monoclonal antibody (Product # 53-9500-42) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

Methylome-based cell-of-origin modeling (Methyl-COOM) identifies aberrant expression of immune regulatory molecules in CLL.

Genome medicine

Wierzbinska JA,Toth R,Ishaque N,Rippe K,Mallm JP,Klett LC,Mertens D,Zenz T,Hielscher T,Seifert M,Küppers R,Assenov Y,Lutsik P,Stilgenbauer S,Roessner PM,Seiffert M,Byrd J,Oakes CC,Plass C,Lipka DB

Published figure using TIGIT monoclonal antibody (Product # 53-9500-42) in Flow Cytometry

Wed Mar 18 00:00:00 EDT 2020

Risk-associated alterations in marrow T cells in pediatric leukemia.

JCI insight

Bailur JK,McCachren SS,Pendleton K,Vasquez JC,Lim HS,Duffy A,Doxie DB,Kaushal A,Foster C,DeRyckere D,Castellino S,Kemp ML,Qiu P,Dhodapkar MV,Dhodapkar KM

Published figure using TIGIT monoclonal antibody (Product # 53-9500-42) in Flow Cytometry

Thu Aug 20 00:00:00 EDT 2020

A Comprehensive Analysis of Key Immune Checkpoint Receptors on Tumor-Infiltrating T Cells From Multiple Types of Cancer.

Frontiers in oncology

Li X,Wang R,Fan P,Yao X,Qin L,Peng Y,Ma M,Asley N,Chang X,Feng Y,Hu Y,Zhang Y,Li C,Fanning G,Jones S,Verrill C,Maldonado-Perez D,Sopp P,Waugh C,Taylor S,Mcgowan S,Cerundolo V,Conlon C,McMichael A,Lu S,Wang X,Li N,Dong T

Published figure using TIGIT monoclonal antibody (Product # 53-9500-42) in Flow Cytometry

Sun Jan 10 00:00:00 EST 2021

TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.

Oncoimmunology

Catakovic K,Gassner FJ,Ratswohl C,Zaborsky N,Rebhandl S,Schubert M,Steiner M,Gutjahr JC,Pleyer L,Egle A,Hartmann TN,Greil R,Geisberger R

Published figure using TIGIT monoclonal antibody (Product # 53-9500-42) in Flow Cytometry

Sat Jan 29 00:00:00 EST 2022

TIGIT Marks Exhausted T Cells, Correlates with Disease Progression, and Serves as a Target for Immune Restoration in HIV and SIV Infection.

PLoS pathogens

Chew GM,Fujita T,Webb GM,Burwitz BJ,Wu HL,Reed JS,Hammond KB,Clayton KL,Ishii N,Abdel-Mohsen M,Liegler T,Mitchell BI,Hecht FM,Ostrowski M,Shikuma CM,Hansen SG,Maurer M,Korman AJ,Deeks SG,Sacha JB,Ndhlovu LC

Published figure using TIGIT monoclonal antibody (Product # 53-9500-42) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Effect of chorioamnionitis on regulatory T cells in moderate/late preterm neonates.

Human immunology

Rueda CM,Wells CB,Gisslen T,Jobe AH,Kallapur SG,Chougnet CA

Published figure using TIGIT monoclonal antibody (Product # 53-9500-42) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

生物信息学

蛋白别名: T cell immunoreceptor with Ig and ITIM domains; T-cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain containing 9; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; Washington University cell adhesion molecule

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基因别名: TIGIT; VSIG9; VSTM3; WUCAM

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UniProt ID: (Human) Q495A1

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Entrez Gene ID: (Human) 201633

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功能

receptor activity receptor binding protein binding protein homodimerization activity cell adhesion molecule binding

参与通路

homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules cell recognition negative regulation of interleukin-12 production positive regulation of interleukin-10 production negative regulation of T cell activation