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TIGIT Antibody (740010MP555) in Flow
TIGIT expression was assessed within CD19 negative (CD19-) and CD19 positive (CD19+) subsets of human PBMCs by staining the cells with CD19 eFluor™ 450 (Product # 48-0199-42) and 0.25 µg of TIGIT Recombinant Mouse Monoclonal Antibody (MBSA43), Alexa Fluor™ Plus 555 (Product # 7400010MP555). Specific TIGIT staining was observed in CD19- subset (pink histogram) while negligible TIGIT staining was seen in the CD19+ subset (blue histogram). Viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD™ Fixable Far Red ... View More
产品信息
740010MP555
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
MBSA43
偶联物
激发/发射光谱
558/572 nm
查看光谱
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein A/G
保存液
proprietary buffer, pH 6.5
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
RRID
AB_2942259
产品详细信息
Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
Obtain superior images with the new Invitrogen™ Alexa Fluor™ Plus antibodies. Make your low abundant targets visible, minimize time spent optimizing, and make your precious samples count. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning images.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.
Applications Tested: This MBSA43 antibody has been tested by flow cytometric analysis of human PBMCs. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: T cell immunoreceptor with Ig and ITIM domains; T-cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain containing 9; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; Washington University cell adhesion molecule
基因别名: TIGIT; VSIG9; VSTM3; WUCAM
UniProt ID: (Human) Q495A1
Entrez Gene ID: (Human) 201633
