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TIMP1 Antibody (PA5-47778) in WB
Western blot was performed using Anti-TIMP1 Polyclonal Antibody (Product # PA5-47778) and a 28 kDa band corresponding to TIMP1 was observed in MDA-MB-231 treated with protein transport inhibitor in comparison to Daudi treated with protein transport inhibitor which is reported to be negative. Whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MDA-MB-231 treated with PTI for 4 hr (Lane 2), Daudi (Lane 3), Daudi treated with PTI for 4 hr (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Reso... View More
产品信息
PA5-47778
产品规格
种属反应
Human,
Rat
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Mouse myeloma cell line NS0-derived recombinant rat TIMP-1 Cys24-Ala217
偶联物
Unconjugated
形式
Lyophilized
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2609009
产品详细信息
In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) TIMP-1 and recombinant mouse TIMP-1 is observed and less than 1% cross-reactivity with rhTIMP-2, rhTIMP-3, and rhTIMP-4 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
靶标信息
TIMP1 (metalloproteinase inhibitor 1) is a metalloproteinase inhibitor that functions by forms one-to-one complexes with target metallproteinases, such as collagenases, and irreversible inactivates them by binding to their catalytic zinc cofactor. TIMP1 acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP14, and MMP16. It also functions as a growth factor that regulates cell differenation, migration, and cell death. TIMP1 activates cellular signaling cascades via CD63 and ITGB1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Collagenase inhibitor; EPA; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; Metalloproteinase inhibitor; Metalloproteinase inhibitor 1; MMP inhibitor; RP1-230G1.3; TIMP 1; TIMP-1; tissue inhibitor of metallopeptidase 1; tissue inhibitor of metalloproteinase 1; Tissue inhibitor of metalloproteinases 1
基因别名: CLGI; EPA; EPO; HCI; TIMP; TIMP-1; TIMP1
UniProt ID: (Human) P01033, (Rat) P30120
Entrez Gene ID: (Human) 7076, (Rat) 116510
cell activation
platelet degranulation
aging
positive regulation of cell proliferation
response to hormone
negative regulation of endopeptidase activity
extracellular matrix disassembly
response to cytokine
wound healing
negative regulation of apoptotic process
negative regulation of catalytic activity
response to peptide hormone
negative regulation of membrane protein ectodomain proteolysis
cartilage development
negative regulation of trophoblast cell migration
regulation of integrin-mediated signaling pathway
steroid biosynthetic process
response to organic substance
