Proteintech

TMEM119 Polyclonal Antibody

TMEM119 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TMEM119 Antibody (27585-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue slide using (Product # 27585-1-AP) TMEM119 antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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产品信息

27585-1-AP

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:800

免疫细胞化学 (ICC/IF)

查看7篇已发表文章 7篇已发表文章

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

TMEM119 Fusion Protein Ag26269 (119-218 aa encoded by NM_181724)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.33 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

产品详细信息

27585-1-AP recognizes a 45-50 kDa band likely due to glycosylation.

Immunogen sequence: MRQKQKASA YYPSSFPKKK YVDQSDRAGG PRAFSEVPDR APDSRPEEAL DSSRQLQADI LAATQNLKSP TRAALGGGDG ARMVEGRGAE EEEKGSQEGD QE (119-218 aa encoded by NM_181724)

靶标信息

Tmem119 encodes a single-pass type I transmembrane protein which seems to interact with a multitude of micro-RNAs as well as certain transcription factors such as SMAD1, SMAD5 and RUNX2. Tmem119 has been identified as a surface marker of microglia that can be used to reliably distinguish microglia from infiltrating macrophages. Microglial cells are phagocytes that are restricted to the brain and involved in clearing up debris and dendrite pruning of neurons. They display a high functional plasticity, balancing neuroprotective and neurotoxic functions, which is reflected by their distinct physiology in response to changes in their microenvironment. Microglia are particularly mobile in areas of the brain undergoing restructuring. Reactive microglia co-express the marker Iba1. In a pathological context, microglia have been associated with differentiation of pathogenic/inflammatory A1 type astrocytes by secretion of Cq1, IL-1a and TNF when stimulated with LPS, and thought to contribute to the pathology of several neurodegenerative diseases such as Alzheimers, Parkinsons, Chorea Huntingtons disease and Multiple Sclerosis. The specific function Tmem119 plays on microglial cells remains unclear. Outside of the neural system, Tmem119 seems to be involved in osteoblast differentiation/ossification.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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