Invitrogen

TNF alpha Polyclonal Antibody

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TNF alpha Antibody (PBOTNFAI) in ELISA

ELISA analysis of TNF-alpha was performed by coating 100 µL per well of anti-bovine TNF-alpha polyclonal antibody (Product # PBOTNFAI) at a concentration of 10 µg/mL and incubating overnight at 4C. The plate was blocked for one hour using 3% bovine serum albumin (BSA) diluted in PBST. The plate was washed six times with 200 µL/well PBST, and then incubated with 100 µL per well of recombinant bovine TNF-alpha protein standards and recombinant ovine TNF-alpha in tissue culture supernatant (both Log2 dilutions starting at an unknown concentra... View More

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产品信息

PBOTNFAI

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

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酶联免疫吸附实验 (ELISA)

Assay-dependent

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产品规格

种属反应

Bovine, Sheep

已发表种属

Bovine, Sheep

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

TNF alpha

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_417104

产品详细信息

PBOTNFAI targets TNF Alpha in ELISA, and WB applications and shows reactivity with bovine and ovine samples.

The PBOTNFAI immunogen is tNF alpha.

PBOTNFAI detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa.

Endotoxin levels are less than or equal to 10 µg/mL.

靶标信息

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Maternal Overnutrition During Gestation in Sheep Alters Autophagy Associated Pathways in Offspring Heart.

Frontiers in genetics

Liu Y,Ding Q,Halderson SJ,Arriola Apelo SI,Jones AK,Pillai SM,Hoffman ML,Reed S,Govoni KE,Zinn SA,Guo W

PBOTNFAI was used in Western Blot to suggest that targeting on autophagy pathway could be a strategy for correction of adverse effects in offspring of over-fed ewes.

Wed Jun 14 00:00:00 EDT 2023

Maternal Overnutrition During Gestation in Sheep Alters Autophagy Associated Pathways in Offspring Heart.

Frontiers in genetics

Liu Y,Ding Q,Halderson SJ,Arriola Apelo SI,Jones AK,Pillai SM,Hoffman ML,Reed S,Govoni KE,Zinn SA,Guo W

PBOTNFAI was used in Western Blot to suggest that targeting on autophagy pathway could be a strategy for correction of adverse effects in offspring of over-fed ewes.

Wed Jun 14 00:00:00 EDT 2023

High-Throughput Production of Chromium(III) Complexes for Antibody Immobilization.

Analytical chemistry

Welch NG,Scoble JA,Easton CD,Williams CC,Bradford BJ,Mamedova LK,Pigram PJ,Muir BW

PBOTNFAI was used in ELISA to use chromium(III) complexes to improve the detection of TNF by ELISA

Tue Oct 18 00:00:00 EDT 2016

Technical note: validation of an ELISA for measurement of tumor necrosis factor alpha in bovine plasma.

Journal of dairy science

Farney JK,Mamedova LK,Godsey BH,Bradford BJ

PBOTNFAI was used in an ELISA assay to develop an effective, low-cost sandwich ELISA procedure for the measurement of tumour necrosis factor alpha in bovine plasma, with improved sensitivity.

Fri Jul 01 00:00:00 EDT 2011

Activation of NF-κB pathway and TNF-α are involved in the cytotoxicity of anthrax lethal toxin in bovine BoMac macrophages.

Veterinary microbiology

Liang X,Gao CF,Rutherford MS,Ji Y

PBOTNFAI was used in western blot to investigate the involvement of the NF-kB pathway and TNF-alpha in the cytotoxicity of anthrax lethal toxin

Sat Nov 20 00:00:00 EST 2010

生物信息学

蛋白别名: Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2

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基因别名: TNF; TNF-a; TNF-alpha; TNFA; TNFSF2

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UniProt ID: (Bovine) A4IFT5, (Sheep) P23383

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Entrez Gene ID: (Bovine) 280943, (Sheep) 443540

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