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Invitrogen
TOMM20 Recombinant Rabbit Monoclonal Antibody (3G5)
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 12
TOMM20 Antibody (MA5-24859) in ICC/IF
Immunofluorescence analysis was performed using Anti-TOMM20 Rabbit Monoclonal Antibody (Product # MA5-24859) using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TOMM20 Rabbit Monoclonal Antibody (Product # MA5-24859) at 1:100 dilution in 0.1% BSA and incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain), Supe... View More
产品信息
MA5-24859
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
3G5
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOMM20.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 50% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2717297
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Purity is > 95% by SDS-PAGE.
靶标信息
The mitochondrial preprotein translocases of the outer membrane (Tom) is a multisubunit protein complex that facilitates the import of nucleus-encoded precursor proteins across the mitochondrial outer membrane. The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria. The import receptors include Tom20 and Tom22, which form a heteromeric receptor complex that initiates the insertion of newly synthesized proteins into the outer membrane and then directs the precursor protein into the GIP. In yeast, Tom22 is the essential component of the import receptor complex as it functions as both a receptor for the preproteins and serves as a docking point for both Tom20 and the GIP.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Mitochondrial 20 kDa outer membrane protein; Mitochondrial import receptor subunit TOM20 homolog; outer mitochondrial membrane receptor rTOM20; Outer mitochondrial membrane receptor Tom20; translocase of outer mitochondrial membrane 20 homolog type II
基因别名: 1810060K07Rik; BB284719; Gm19268; KIAA0016; MAS20; mKIAA0016; MOM19; TOM20; TOMM20
UniProt ID: (Human) Q15388, (Rat) Q62760, (Mouse) Q9DCC8
Entrez Gene ID: (Human) 9804, (Rat) 266601, (Mouse) 67952
