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TPH2 Antibody (702017) in ICC/IF
Immunofluorescence analysis of Tryptophan 5-hydroxylase 2 was performed using 70 confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TPH2 Recombinant Rabbit Monoclonal Antibody (9H1L6) (Product # 702017) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody,... View More
产品信息
702017
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
9H1L6
抗原
Protein corresponding to Human TPH2 (aa 1-160)
偶联物
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2633049
产品详细信息
This antibody is predicted to react with Monkey, Sheep and Rat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Tryptophan hydroxylase (TPH) is a member of the pterin-dependent aromatic acid hydroxylase family. It catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. TPH is expressed predominantly in the brain stem. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: MGC119994; Neuronal tryptophan hydroxylase; TPRH; TRPH; Tryptophan 5-hydroxylase 2; Tryptophan 5-monooxygenase 2
基因别名: ADHD7; AU043594; NTPH; TPH2
UniProt ID: (Human) Q8IWU9, (Mouse) Q8CGV2, (Rat) Q8CGU9
Entrez Gene ID: (Human) 121278, (Mouse) 216343, (Rat) 317675
tryptophan 5-monooxygenase activity
iron ion binding
amino acid binding
monooxygenase activity
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen
metal ion binding
molecular_function
circadian rhythm
aromatic amino acid family metabolic process
response to activity
response to nutrient levels
serotonin biosynthetic process
response to estrogen
indolalkylamine biosynthetic process
response to glucocorticoid
response to calcium ion
oxidation-reduction process
cellular response to lithium ion
serotonin biosynthetic process from tryptophan
metabolic process
