Invitrogen

TRA-1-81 (Podocalyxin) Monoclonal Antibody (TRA-1-81), PE, eBioscience™

TRA-1-81 (Podocalyxin) Antibody in Flow Cytometry (Flow)

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TRA-1-81 (Podocalyxin) Antibody (12-8883-82) in Flow

Staining of 2102Ep human embryonal carcinoma cell line with 0.5 µg of Mouse IgM Isotype Control PE (blue histogram) or 0.5 µg of Anti-Human TRA-1-81 PE (purple histogram). Total viable cells were used for analysis.

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产品信息

12-8883-82

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

1 µg/test

查看10篇已发表文章 10篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgM

分类

Monoclonal

类型

Antibody

克隆号

TRA-1-81

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_891606

产品详细信息

Description: The TRA-1-81 antibody recognizes a protein expressed on undifferentiated human embryonic stem cells (ES), embyronal carcinoma cells (EC), and embryonic germ cells (EG). Like other stem cell specific markers, the epitope recognized by the TRA-1-81 antibody is lost upon cell differentiation. The TRA-1-81 epitope is resistant to neuraminidase digestion, unlike the epitope recognized by the related TRA-1-60 antibody. The TRA-1-81 antibody is known to specifically recognize a carbohydrate epitope on a keratan sulfated glycoprotein recently identified as podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein originally identified on epithelial glomerular cells known as podocytes, and the protein has also been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.

Applications Reported: This TRA-1-81 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TRA-1-81 antibody has been tested by flow cytometric analysis of the human embryonal carcinoma (EC) line 2102Ep. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

TRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Low-density lipoprotein receptor-deficient hepatocytes differentiated from induced pluripotent stem cells allow familial hypercholesterolemia modeling, CRISPR/Cas-mediated genetic correction, and productive hepatitis C virus infection.

Stem cell research & therapy

Caron J,Pène V,Tolosa L,Villaret M,Luce E,Fourrier A,Heslan JM,Saheb S,Bruckert E,Gómez-Lechón MJ,Nguyen TH,Rosenberg AR,Weber A,Dubart-Kupperschmitt A

12-8883-82 was used in Flow Cytometry to provides the first LDLR-null FH cell model and its corrected counterpart to study the regulation of cholesterol metabolism and host determinants of HCV life cycle, and a platform to screen drugs for treating dyslipidemia and HCV infection.

Mon Jul 29 00:00:00 EDT 2019

Ultra-High-Frequency Reprogramming of Individual Long-Term Hematopoietic Stem Cells Yields Low Somatic Variant Induced Pluripotent Stem Cells.

Cell reports

Wang K,Guzman AK,Yan Z,Zhang S,Hu MY,Hamaneh MB,Yu YK,Tolu S,Zhang J,Kanavy HE,Ye K,Bartholdy B,Bouhassira EE

12-8883-82 was used in Flow Cytometry to suggest LT-HSCs may become the preferred cell source for the production of clinical-grade iPSCs.

Tue Mar 05 00:00:00 EST 2019

Simplified Footprint-Free Cas9/CRISPR Editing of Cardiac-Associated Genes in Human Pluripotent Stem Cells.

Stem cells and development

Kondrashov A,Duc Hoang M,Smith JGW,Bhagwan JR,Duncan G,Mosqueira D,Munoz MB,Vo NTN,Denning C

Published figure using TRA-1-81 (Podocalyxin) monoclonal antibody (Product # 12-8883-82) in Flow Cytometry

Thu Mar 15 00:00:00 EDT 2018

Drug-Mediated Shortening of Action Potentials in LQTS2 Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Stem cells and development

Duncan G,Firth K,George V,Hoang MD,Staniforth A,Smith G,Denning C

12-8883 was used in Flow cytometry/Cell sorting to investigate the importance of drug-mediated shortening of action potentials in cardiomyocytes from patients with LQTS2.

Fri Dec 01 00:00:00 EST 2017

Comparison of Four Protocols to Generate Chondrocyte-Like Cells from Human Induced Pluripotent Stem Cells (hiPSCs).

Stem cell reviews and reports

Suchorska WM,Augustyniak E,Richter M,Trzeciak T

Published figure using TRA-1-81 (Podocalyxin) monoclonal antibody (Product # 12-8883-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting.

Nature communications

Chen X,Janssen JM,Liu J,Maggio I,'t Jong AEJ,Mikkers HMM,Gonçalves MAFV

12-8883 was used in Flow cytometry/Cell sorting to use Cas9 nickases to generate coordinated single-strand breaks in donor and target DNA for precise homology-directed gene editing.

Fri Sep 22 00:00:00 EDT 2017

Comparative neurotoxicity screening in human iPSC-derived neural stem cells, neurons and astrocytes.

Brain research

Pei Y,Peng J,Behl M,Sipes NS,Shockley KR,Rao MS,Tice RR,Zeng X

12-8883 was used in Immunocytochemistry to examine the comparative cytotoxicity of 80 compounds in induced pluripotent stem cells(iPSC), isogenic iPSC-derived neural stem cells, neurons and astrocytes.

Sun May 01 00:00:00 EDT 2016

Generation of functional cholangiocyte-like cells from human pluripotent stem cells and HepaRG cells.

Hepatology (Baltimore, Md.)

Dianat N,Dubois-Pot-Schneider H,Steichen C,Desterke C,Leclerc P,Raveux A,Combettes L,Weber A,Corlu A,Dubart-Kupperschmitt A

Published figure using TRA-1-81 (Podocalyxin) monoclonal antibody (Product # 12-8883-82) in Flow Cytometry

Fri Aug 01 00:00:00 EDT 2014

The ontogeny of cKIT+ human primordial germ cells proves to be a resource for human germ line reprogramming, imprint erasure and in vitro differentiation.

Nature cell biology

Gkountela S,Li Z,Vincent JJ,Zhang KX,Chen A,Pellegrini M,Clark AT

Published figure using TRA-1-81 (Podocalyxin) monoclonal antibody (Product # 12-8883-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2013

Procedures for derivation and characterisation of human embryonic stem cells from Odense, Denmark.

Methods in molecular biology (Clifton, N.J.)

Harkness L,Kassem M

12-8883-82 was used in flow cytometry to describe the derivation methods used by our laboratory for isolation of the ICM by immunosurgery and outgrowth of the whole blastocyst.

Fri Aug 10 00:00:00 EDT 2012

Faster generation of hiPSCs by coupling high-titer lentivirus and column-based positive selection.

Nature protocols

Dick E,Matsa E,Young LE,Darling D,Denning C

12-8883-82 was used in Flow cytometry/Cell sorting to present methods which accelerate the production of hiPSCs and enable lines to be isolated, expanded to approxiamtely 107 cells and cryopreserved within 6-8 weeks.

Wed Jun 01 00:00:00 EDT 2011

生物信息学

蛋白别名: tra1-81; TRA180

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TRA-1-81 (Podocalyxin) Monoclonal Antibody (TRA-1-81), PE, eBioscience™

TRA-1-81 (Podocalyxin) Antibody (12-8883-82) in Flow

产品信息

12-8883-82

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

How to use the Panel Builder

生物信息学

性能保障

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