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TRBC1 Chimeric Antibody (704904) in Flow
Wild type (A) and TRBC1 knockout (B) Jurkat cells were stained with CD3 FITC (Product # 11-0038-42) and 0.25 µg of TRBC1 Recombinant Human-Mouse Chimeric Monoclonal Antibody (Product # 704904) (pink histogram) or left unstained (blue histogram), followed by Goat anti-Mouse IgG (H+L) Alexa Fluor™ Plus 647 (Product # A55060). Staining index (C) was calculated to show the relative intensity of TRBC1 staining in wild type and knockout cells. Viable cells in the lymphocyte gate were used for analysis, as determined by Fixable Viability Dye eFlu... View More
产品信息
704904
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG2a, kappa
Expression System
ExpiCHO
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JOVI.1
偶联物
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_3092871
产品详细信息
Recombinant human-mouse chimeric monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
Constant region of T cell receptor (TR) alpha chain (PubMed:24600447). Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens (PubMed:25493333). Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB) pathways, leading to the mobilization of transcription factors that are critical for gene expression and essential for T cell growth and differentiation (PubMed:9382891, PubMed:23524462). The T cell repertoire is generated in the thymus, by V-(D)-J rearrangement. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MH restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TR with the pMH complexes shapes TR structural and functional avidity (PubMed:15040585). [UniProt]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名: BV05S1J2.2; TCRB; TCRBC1
Entrez Gene ID: (Human) 28639
