Search Thermo Fisher Scientific
- Contact Us
- Quick Order
-
Don't have an account ? Create Account
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Application
FIGURE: 1 / 1
TRIM2 Antibody (PA5-89800) in WB
Western blot analysis of TRIM2 in extracts of mouse small intestine. Samples were incubated with TRIM2 Polyclonal antibody (Product # PA5-89800) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Product Details
PA5-89800
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human TRIM2 (NP_0560862)
Conjugate
Unconjugated
Form
Liquid
Concentration
2.13 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805781
Product Specific Information
Immunogen sequence: GVAALQNNFF ITNLMDVLQR TPGSNAEESS ILETVTAVAA GKPLSCPNHD GNVMEFYCQS CETAMCRECT EGEHAEHPTV PLKDVVEQHK ASLQVQLDAV NKRLPEIDSA LQFISEIIHQ LTNQKASIVD DIHSTFDELQ KTLNVRKSVL LMELEVNYGL KHKVLQSQLD TLLQGQESIK SCSNFTAQAL NHGTETEVLL V; Positive Samples: Mouse small intestine; Cellular Location: Cytoplasm
Target Information
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. Its function has not been identified.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase TRIM2; Neural activity-related RING finger protein; RING-type E3 ubiquitin transferase TRIM2; tripartite motif protein 2; tripartite motif protein TRIM2; Tripartite motif-containing protein 2
Gene Aliases: Kiaa0517; mKIAA0517; Narf; Trim2
UniProt ID: (Mouse) Q9ESN6, (Rat) D3ZQG6
Entrez Gene ID: (Mouse) 80890, (Rat) 361970
ubiquitin-protein transferase activity
protein binding
zinc ion binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
myosin binding
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
ubiquitin protein ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support