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Invitrogen
TRIM28 Polyclonal Antibody
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
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TRIM28 Antibody (PA5-27648) in ICC/IF
Immunofluorescence analysis of TRIM28 was performed using 70 confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TRIM28 Polyclonal Antibody (Product # PA5-27648) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32... View More
产品信息
PA5-27648
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 361 and 613 of Human KAP1
偶联物
形式
Liquid
浓度
0.48 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol, 1% BSA
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2545124
产品详细信息
Recommended positive controls: H1299, HeLa, NIH-3T3.
Predicted reactivity: Mouse (91%), Rat (91%), Bovine (93%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. There have been identified over 220 KRAB-zinc finger protein (KRAB-ZFP) genes in the human genome. These proteins functionally repress transcription via specific interactions with KAP-1 (KRAB-associated protein 1). KAP-1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP-1 has been shown to form complexes with KRAB-domain transcription factors and increase the efficiency with which they mediate repression. KAP-1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, thus helping to regulate transcriptional repression. Studies have shown that KAP-1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: E3 SUMO-protein ligase TRIM28; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-A-interacting protein; KRAB-associated protein 1; KRAB-interacting p; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; protein phosphatase 1, regulatory subunit 157; RING finger protein 96; RING-type E3 ubiquitin transferase TIF1-beta; tif1 beta; TIF1-beta; Transcription intermediary factor 1-beta; transcriptional intermediary factor 1, beta; transcriptional intermediary factor 1-beta; tripartite motif; tripartite motif protein 28; Tripartite motif-containing protein 28
基因别名: AA408787; KAP-1; KAP1; KRIP-1; Krip1; MommeD9; PPP1R157; RNF96; TF1B; TIF1B; Tif1beta; TRIM28
UniProt ID: (Human) Q13263, (Mouse) Q62318, (Rat) O08629
Entrez Gene ID: (Human) 10155, (Mouse) 21849, (Rat) 116698
RNA polymerase II transcription coactivator activity
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein kinase activity
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
ubiquitin protein ligase binding
Krueppel-associated box domain binding
sequence-specific DNA binding
poly(A) RNA binding
chromo shadow domain binding
promoter-specific chromatin binding
transcription coactivator activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
epithelial to mesenchymal transition
DNA repair
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
embryo implantation
protein ubiquitination
protein sumoylation
positive regulation of transcription factor import into nucleus
DNA methylation involved in embryo development
innate immune response
positive regulation of DNA repair
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
protein autophosphorylation
protein oligomerization
convergent extension involved in axis elongation
embryonic placenta morphogenesis
negative regulation of DNA demethylation
negative regulation of viral release from host cell
regulation of genetic imprinting
transcription initiation from RNA polymerase II promoter
Ras protein signal transduction
covalent chromatin modification
positive regulation of transcription from RNA polymerase II promoter
positive regulation of methylation-dependent chromatin silencing
