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产品信息
MA5-44223
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
BL-248-2G6
抗原
Synthetic peptide representing a portion of human KRAB-Associated Protein 1 surrounding Serine 824.
偶联物
形式
Liquid
浓度
40 µg/mL
纯化类型
purified
保存液
BBS, pH 7.8-8.6, with 0.1% BSA
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Wet ice
RRID
AB_2926353
产品详细信息
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
靶标信息
The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. There have been identified over 220 KRAB-zinc finger protein (KRAB-ZFP) genes in the human genome. These proteins functionally repress transcription via specific interactions with KAP-1 (KRAB-associated protein 1). KAP-1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP-1 has been shown to form complexes with KRAB-domain transcription factors and increase the efficiency with which they mediate repression. KAP-1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, thus helping to regulate transcriptional repression. Studies have shown that KAP-1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.
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生物信息学
蛋白别名: E3 SUMO-protein ligase TRIM28; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-associated protein 1; KRAB-interacting p; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; protein phosphatase 1, regulatory subunit 157; RING finger protein 96; RING-type E3 ubiquitin transferase TIF1-beta; tif1 beta; TIF1-beta; Transcription intermediary factor 1-beta; transcriptional intermediary factor 1-beta; tripartite motif; Tripartite motif-containing protein 28
基因别名: KAP1; PPP1R157; RNF96; TF1B; TIF1B; TRIM28
UniProt ID: (Human) Q13263
Entrez Gene ID: (Human) 10155
RNA polymerase II transcription coactivator activity
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein kinase activity
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
ubiquitin protein ligase binding
Krueppel-associated box domain binding
sequence-specific DNA binding
poly(A) RNA binding
chromo shadow domain binding
promoter-specific chromatin binding
negative regulation of transcription from RNA polymerase II promoter
epithelial to mesenchymal transition
DNA repair
transcription initiation from RNA polymerase II promoter
Ras protein signal transduction
embryo implantation
protein ubiquitination
covalent chromatin modification
protein sumoylation
positive regulation of transcription factor import into nucleus
DNA methylation involved in embryo development
innate immune response
positive regulation of DNA repair
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
protein autophosphorylation
protein oligomerization
convergent extension involved in axis elongation
embryonic placenta morphogenesis
positive regulation of methylation-dependent chromatin silencing
negative regulation of DNA demethylation
negative regulation of viral release from host cell
regulation of genetic imprinting
