Invitrogen

TRPM7 Monoclonal Antibody (S74)

TRPM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TRPM7 Antibody (MA5-27620) in IHC (P)

Immunohistochemistry analysis of TRPM7 in paraffin-embedded mouse hippocampus. Samples were incubated with TRPM7 monoclonal antibody (Product # MA5-27620) using a dilution of 1:1000 (1 hour at RT) and followed by Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) and Mayer Hematoxylin (purple/blue) at a dilution of 250-500 µl.

TRPM7 Antibody in Immunocytochemistry (ICC/IF)

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产品信息

MA5-27620

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:1,000

免疫细胞化学 (ICC/IF)

1:100

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免疫沉淀 (IP)

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Antibody Microarray (AM)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse, Pig, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

S74

抗原

Fusion protein amino acids 1817-1863 (C- terminus) of mouse TrpM7

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2735401

产品详细信息

1 µg/mL of MA5-27620 was sufficient for detection of TrpM7 in 10 µg of COS cell lysate transiently transfected with TprM7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 220kDa in extracts of cells transiently transfected with TrpM7. No cross-reactivity against TrpM6.

靶标信息

TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Detection of TRPM6 and TRPM7 Proteins in Normal and Diseased Cardiac Atrial Tissue and Isolated Cardiomyocytes.

International journal of molecular sciences

Andriulė I,Pangonytė D,Gwanyanya A,Karčiauskas D,Mubagwa K,Mačianskienė R

MA5-27620 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to study the expression patterns of TRPM6 and TRPM7 in normal and diseased myocardium.

Mon Nov 28 00:00:00 EST 2022

The molecular appearance of native TRPM7 channel complexes identified by high-resolution proteomics.

eLife

Kollewe A,Chubanov V,Tseung FT,Correia L,Schmidt E,Rössig A,Zierler S,Haupt A,Müller CS,Bildl W,Schulte U,Nicke A,Fakler B,Gudermann T

MA5-27620 was used in Immunoprecipitation to investigate the subunit composition of endogenous TRPM7 channels in rodent brain by multi-epitope affinity purification and high-resolution quantitative mass spectrometry analysis.

Fri Nov 12 00:00:00 EST 2021

Epidermal growth factor signaling through transient receptor potential melastatin 7 cation channel regulates vascular smooth muscle cell function.

Clinical science (London, England : 1979)

Zou ZG,Rios FJ,Neves KB,Alves-Lopes R,Ling J,Baillie GS,Gao X,Fuller W,Camargo LL,Gudermann T,Chubanov V,Montezano AC,Touyz RM

MA5-27620 was used in Immunocytochemistry to determine whether TRPM7 is a downstream target of EGF in VSMCs and if EGF receptor through TRPM7 influences VSMC function.

Fri Aug 14 00:00:00 EDT 2020

Epidermal growth factor signaling through transient receptor potential melastatin 7 cation channel regulates vascular smooth muscle cell function.

Clinical science (London, England : 1979)

Zou ZG,Rios FJ,Neves KB,Alves-Lopes R,Ling J,Baillie GS,Gao X,Fuller W,Camargo LL,Gudermann T,Chubanov V,Montezano AC,Touyz RM

MA5-27620 was used in Immunocytochemistry to determine whether TRPM7 is a downstream target of EGF in VSMCs and if EGF receptor through TRPM7 influences VSMC function.

Fri Aug 14 00:00:00 EDT 2020

生物信息学

蛋白别名: Channel kinase 1; Channel-kinase 1; FLJ20117; Long transient receptor potential channel 7; long transient receptor potential channel 7 {ECO:0000250|UniProtKB:Q96QT4}; LTRPC ion channel family member 7; LTrpC-7; LTrpC7 {ECO:0000250|UniProtKB:Q96QT4}; tr; Transient receptor potential cation channel subfamily M member 7; transient receptor potential cation channel subfamily M member 7 {ECO:0000312|RGD:620053}; transient receptor potential cation channel, subfamily M, member 7; transient receptor potential M7; Transient receptor potential-phospholipase C-interacting kinase; transient receptor potential-phospholipase C-interacting kinase {ECO:0000303|PubMed:11161216}; transient receptor potential-related protein, ChaK; TRMP7; TRP PLIK; TRP-PLIK; TRP-PLIK {ECO:0000303|PubMed:11161216}; trpm7 {ECO:0000312|RGD:620053}

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基因别名: 2310022G15Rik; 4833414K03Rik; 5033407O22Rik; ALSPDC; CHAK; CHAK1; Ltpr7; LTrpC-7; LTRPC7; TRP-PLIK; TRPM7; TRPPLIK

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UniProt ID: (Human) Q96QT4, (Mouse) Q925B2

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Entrez Gene ID: (Human) 54822, (Mouse) 58800, (Rat) 679906

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功能