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TRPV6 Antibody (PA5-141059) in ICC/IF
Immunocytochemistry analysis of TRPV6 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Samples were incubated in TRPV6 Polyclonal antibody (Product # PA5-141059) using a dilution of 1:100 for 1 hour at room temperature, washed with PBS followed by Alexa Fluor®488 Goat anti-Rabbit IgG secondary antibody at a dilution of 1:100. The nuclear counter stain is DAPI (blue).
产品信息
PA5-141059
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to C terminal of Human TRPV6 .
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4, with 0.05% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2932511
靶标信息
Calcium-permeable channels, such as TRPV6, participate in neurotransmission, muscle contraction, and exocytosis by providing calcium as an intracellular second messenger. Depending on the tissue, transcellular calcium transport may be regulated by vitamin D, parathyroid hormone (PTH), or calcitonin (CALCA). Calcium selective cation channel probably involved in Ca(2+) uptake in various tissues, including Ca(2+) reabsorption in intestine. The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification. Inactivation includes both, a rapid Ca(2+)-dependent and a slower Ca(2+)-calmodulin-dependent mechanism, the latter may be regulated by phosphorylation. In vitro, is slowly inhibited by Mg(2+) in a voltage-independent manner. Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating. Interacts with TRPV5. Interacts with S100A10 and probably with the ANAX2-S100A10 heterotetramer. The interaction with S100A10 is required for the trafficking to the plasma membrane. Interacts with BSPRY. Interacts with calmodulin.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Alu-binding protein with zinc finger domain; Calcium transport protein 1; calcium transporter-like protein; CaT-L; CaT-like; CaT1; ECaC2; epithelial apical membrane calcium transporter/channel CaT1; Epithelial calcium channel 2; osmosensitive transient receptor potential channel 3; Transient receptor potential cation channel subfamily V member 6; transient receptor potential cation channel, subfamily 5, member 6; TrpV6
基因别名: ABP/ZF; Cac; CAT; CAT1; CATL; ECAC2; HSA277909; LP6728; Otrpc3; TRPV6; ZFAB
UniProt ID: (Human) Q9H1D0, (Rat) Q9R186, (Mouse) Q91WD2
Entrez Gene ID: (Human) 55503, (Rat) 114246, (Mouse) 64177
