Invitrogen

TSC2 Monoclonal Antibody (3G9D9)

TSC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 10

TSC2 Antibody (37-0500) in IHC (P)

Immunohistochemistry analysis of Tuberin/TSC2 showing staining in the cytoplasm of paraffin-embedded human liver tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperatu... View More

TSC2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

TSC2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

产品信息

37-0500

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

查看4篇已发表文章 4篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

3G9D9

抗原

Recombinant human tuberin protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533293

靶标信息

TSC2 (Tuberin, Tuberous sclerosis complex, TSC Complex Subunit 2), is implicated as a tumor suppressor and may function in vesicular transport, play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. TSC2 associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Phosphorylation of tuberin on Ser 939 and Thr 1462 regulates its interaction with hamartin, and is stimulated by various growth factors through the phosphoinositide 3-kinase/Akt pathway. TSC2 may have a function in vesicular transport, but may also play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. Interaction between TSC1 and TSC2 may facilitate vesicular docking. TSC2 specifically stimulates the intrinsic GTPase activity of the Ras related protein RAP1A and RAB5, suggesting a possible mechanism for its role in regulating cellular growth. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors and are involved in diseases such as lymphangioleiomyomatosis and TSC2 angiomyolipoma. Alternative splicing results in transcript variants of three different isoforms of TSC2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

G3BPs tether the TSC complex to lysosomes and suppress mTORC1 signaling.

Cell

Prentzell MT,Rehbein U,Cadena Sandoval M,De Meulemeester AS,Baumeister R,Brohée L,Berdel B,Bockwoldt M,Carroll B,Chowdhury SR,von Deimling A,Demetriades C,Figlia G,de Araujo MEG,Heberle AM,Heiland I,Holzwarth B,Huber LA,Jaworski J,Kedra M,Kern K,Kopach A,Korolchuk VI,van 't Land-Kuper I,Macias M,Nellist M,Palm W,Pusch S,Ramos Pittol JM,Reil M,Reintjes A,Reuter F,Sampson JR,Scheldeman C,Siekierska A,Stefan E,Teleman AA,Thomas LE,Torres-Quesada O,Trump S,West HD,de Witte P,Woltering S,Yordanov TE,Zmorzynska J,Opitz CA,Thedieck K

37-0500 was used in Immunoprecipitation to report that Ras GTPase-activating protein-binding proteins 1 and 2 reside at the cytoplasmic surface of lysosomes.

Thu Feb 04 00:00:00 EST 2021

G3BPs tether the TSC complex to lysosomes and suppress mTORC1 signaling.

Cell

37-0500 was used in Immunoprecipitation to report that Ras GTPase-activating protein-binding proteins 1 and 2 reside at the cytoplasmic surface of lysosomes.

Thu Feb 04 00:00:00 EST 2021

The splicing factor U2AF1 contributes to cancer progression through a noncanonical role in translation regulation.

Genes & development

Palangat M,Anastasakis DG,Fei DL,Lindblad KE,Bradley R,Hourigan CS,Hafner M,Larson DR

37-0500 was used in Immunocytochemistry-immunoflourescence to show a noncanonical function of U2AF1 whereby it can directly bind mature mNA in the cytoplasm and negatively regulate mRNA translation, contributing to cancer progression.

Wed May 01 00:00:00 EDT 2019

Death-associated protein kinase 1 promotes growth of p53-mutant cancers.

The Journal of clinical investigation

Zhao J,Zhao D,Poage GM,Mazumdar A,Zhang Y,Hill JL,Hartman ZC,Savage MI,Mills GB,Brown PH

37-0500 was used in immunoprecipitation to identify molecular targets that suppress tumorigenesis in triple negative breast cancers.

Wed Jul 01 00:00:00 EDT 2015

Death-associated protein kinase 1 promotes growth of p53-mutant cancers.

The Journal of clinical investigation

Zhao J,Zhao D,Poage GM,Mazumdar A,Zhang Y,Hill JL,Hartman ZC,Savage MI,Mills GB,Brown PH

37-0500 was used in immunoprecipitation to identify molecular targets that suppress tumorigenesis in triple negative breast cancers.

Wed Jul 01 00:00:00 EDT 2015

Spatial control of the TSC complex integrates insulin and nutrient regulation of mTORC1 at the lysosome.

Cell

Menon S,Dibble CC,Talbott G,Hoxhaj G,Valvezan AJ,Takahashi H,Cantley LC,Manning BD

Published figure using TSC2 monoclonal antibody (Product # 37-0500) in Immunoprecipitation

Thu Feb 13 00:00:00 EST 2014

TBC1D7 is a third subunit of the TSC1-TSC2 complex upstream of mTORC1.

Molecular cell

Dibble CC,Elis W,Menon S,Qin W,Klekota J,Asara JM,Finan PM,Kwiatkowski DJ,Murphy LO,Manning BD

Fri Aug 24 00:00:00 EDT 2012

TBC1D7 is a third subunit of the TSC1-TSC2 complex upstream of mTORC1.

Molecular cell

Dibble CC,Elis W,Menon S,Qin W,Klekota J,Asara JM,Finan PM,Kwiatkowski DJ,Murphy LO,Manning BD

Fri Aug 24 00:00:00 EDT 2012

Clinicopathological and immunohistochemical findings in an autopsy case of tuberous sclerosis complex.

Neuropathology : official journal of the Japanese Society of Neuropathology

Boer K,Troost D,Jansen F,Nellist M,van den Ouweland AM,Geurts JJ,Spliet WG,Crino P,Aronica E

37-0500 was used in immunohistochemistry - paraffin section to report the neuropathological findings of a 32-year-old patient with a germ-line mutation in the TSC2 gene