Invitrogen

TSG101 Recombinant Rabbit Monoclonal Antibody (JJ0900)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2个已发表图片

2 篇参考文献

查看其他25个TSG101抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

TSG101 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 15

TSG101 Antibody (MA5-32463) in ICC/IF

Immunofluorescence analysis of Tsg101 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TSG101 Recombinant Rabbit Monoclonal Antibody (JJ0900) (Product # MA5-32463, 1:100) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488... View More

TSG101 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TSG101 Antibody in Flow Cytometry (Flow)

Product Image Gallery

产品信息

MA5-32463

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:500

流式细胞分析 (Flow)

1:1,000

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JJ0900

抗原

Synthetic peptide within Human TSG101 aa 24-73

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2809740

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

TSG101-4A10 recognizes TSG101 protein, the product of a recently identified Tumor Susceptibility Gene whose inactivation in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice. The TSG101 gene encodes a 46 kDa protein and contains a proline-rich domain and a leucine-zipper motif within a coiled-coil domain near the carboxyl terminus.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Trojan horse treatment based on PEG-coated extracellular vesicles to deliver doxorubicin to melanoma in vitro and in vivo.

Cancer biology & therapy

Patras L,Ionescu AE,Munteanu C,Hajdu R,Kosa A,Porfire A,Licarete E,Rauca VF,Sesarman A,Luput L,Bulzu P,Chiroi P,Tranca RA,Meszaros MS,Negrea G,Barbu-Tudoran L,Potara M,Szedlacsek S,Banciu M

MA5-32463 was used in Western Blotting to highlight the promising potential of EVs as optimal tools for systemic delivery of DOX to solid tumors.

Sat Dec 31 00:00:00 EST 2022

Trojan horse treatment based on PEG-coated extracellular vesicles to deliver doxorubicin to melanoma in vitro and in vivo.

Cancer biology & therapy

Patras L,Ionescu AE,Munteanu C,Hajdu R,Kosa A,Porfire A,Licarete E,Rauca VF,Sesarman A,Luput L,Bulzu P,Chiroi P,Tranca RA,Meszaros MS,Negrea G,Barbu-Tudoran L,Potara M,Szedlacsek S,Banciu M

MA5-32463 was used in Western Blotting to highlight the promising potential of EVs as optimal tools for systemic delivery of DOX to solid tumors.

Sat Dec 31 00:00:00 EST 2022

The protective effects of MSC-EXO against pulmonary hypertension through regulating Wnt5a/BMP signalling pathway.

Journal of cellular and molecular medicine

Zhang Z,Ge L,Zhang S,Wang J,Jiang W,Xin Q,Luan Y

MA5-32463 was used in Western Blotting to explore the mechanism of mesenchymal stem cell-derived exosomes (MSC-EXO) to protect against experimentally induced pulmonary hypertension (PH).

Tue Dec 01 00:00:00 EST 2020

The protective effects of MSC-EXO against pulmonary hypertension through regulating Wnt5a/BMP signalling pathway.

Journal of cellular and molecular medicine

Zhang Z,Ge L,Zhang S,Wang J,Jiang W,Xin Q,Luan Y

MA5-32463 was used in Western Blotting to explore the mechanism of mesenchymal stem cell-derived exosomes (MSC-EXO) to protect against experimentally induced pulmonary hypertension (PH).

Tue Dec 01 00:00:00 EST 2020

生物信息学

蛋白别名: ESCRT-I complex subunit TSG101; ESCRTI complex subunit TSG101; TSG 101; Tumor supressor; tumor susceptibility gene 10; tumor susceptibility gene 101; Tumor susceptibility gene 101 protein; tumor susceptibility protein

查看更多收起

基因别名: AI255943; CC2; Rw; TSG10; TSG101; VPS23

查看更多收起

UniProt ID: (Human) Q99816, (Rat) Q6IRE4, (Mouse) Q61187

查看更多收起

Entrez Gene ID: (Human) 7251, (Rat) 292925, (Mouse) 22088

查看更多收起

功能

DNA binding transcription corepressor activity protein binding ligand-dependent nuclear receptor transcription coactivator activity ubiquitin protein ligase binding protein homodimerization activity ubiquitin binding virion binding calcium-dependent protein binding ubiquitin-protein ligase

参与通路