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TSLP Antibody (500-P258-50UG) in WB
Western Blot: To detect Human TSLP by Western Blot analysis TSLP Polyclonal Antibody (Product # 500-P258-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TSLP is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P258-50UG
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human TSLP
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930112
产品详细信息
AA Sequence of recombinant protein: MYDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV SCSNRPHCLT EIQSLTFNPTAGCASLAKEM FAMKTKAALA IWCPGYSETQ INATQAMKKR RKRKVTTNKC LEQVSQLQGLWRRFNRPLLK QQ.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human TSLP. Anti-Human TSLP-specific antibody was purified by affinity chromatography employing an immobilized Human TSLP matrix.
Sandwich ELISA: To detect Human TSLP by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human TSLP (500-P258Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hHuman TSLP.
Western Blot: To detect Human TSLP by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TSLP is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
TSLP is a 159 amino acid four helix-bundle hemopoietic cytokine that signals through a heterodimeric receptor complex consisting of the human TSLP receptor and the IL-7R alpha-chain. It impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes, enhances the maturation of CD11c (+) dendritic cells, naive CD4 (+), and CD8 (+) T cells into proallergic effectors, and primes dendritic cells to produce large amounts of IL-12 after CD40 ligand stimulation. TSLP influences the regulation of the positive selection of regulatory T cells and maintenance of peripheral CD4 (+) T cell homeostasis. It induces allergic inflammation by directly activating mast cells and may be involved in the pathophysiology of inflammatory arthritis. It is expressed in heart, liver and prostate.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Thymic stromal lymphopoietin
基因别名: TSLP
UniProt ID: (Human) Q969D9
Entrez Gene ID: (Human) 85480
