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Talin 1 Antibody (MA5-49244) in WB
Western blot analysis of TLN1 using TLN1 antibody (Product # MA5-49244). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human Raji whole cell lysates; Lane 2: human SW620 whole cell lysates; Lane 3: human COLO320 whole cell lysates; Lane 4: human 293T whole cell lysates; Lane 5: rat lung tissue lysates; Lane 6: mouse lung tissue lysates; Lane 7: mouse liver tissue lysates. After e... View More
产品信息
MA5-49244
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
2G10D2
抗原
E.coli-derived human Talin 1/TLN1 recombinant protein (Position: Q1658-N1876).
偶联物
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_3074902
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Raji whole cell, human SW620 whole cell, human COLO320 whole cell, human 293T whole cell, rat lung tissue, mouse lung tissue, mouse liver tissue. Flow: JK cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-235 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Talin-1
基因别名: ILWEQ; KIAA1027; TLN; TLN1
UniProt ID: (Human) Q9NZQ2, (Mouse) P26039
Entrez Gene ID: (Human) 7094, (Rat) 313494, (Mouse) 21894
