Invitrogen

Thymidylate Synthase Recombinant Rabbit Monoclonal Antibody (HL1236)

Thymidylate Synthase Antibody in Immunocytochemistry (ICC/IF)

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Thymidylate Synthase Antibody (MA5-46968) in ICC/IF

Immunofluorescent analysis of Thymidylate Synthase in HeLa cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thymidylate synthase stained by Thymidylate synthase monoclonal antibody (Product # MA5-46968) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1,000. Blue: Fluoroshield with DAPI.

Thymidylate Synthase Antibody in Western Blot (WB)

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产品信息

MA5-46968

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫细胞化学 (ICC/IF)

Assay-dependent

产品规格

种属反应

Dog, Cat, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

HL1236

抗原

Recombinant protein encompassing a sequence within the center region of human Thymidylate synthase. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2938040

产品详细信息

Store as concentrated solution.

Centrifuge briefly prior to opening vial.

靶标信息

Thymidylate Synthase (TS) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

仅用于科研。不用于诊断过程。未经明确授权不得转售。