Zeta

Thyroglobulin Antibody Cocktail

Thyroglobulin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Thyroglobulin Antibody (Z2062MP) in IHC (P)

Human thyroid gland stained with anti-thyroglobulin antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic of follicular cells.

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产品信息

Z2062MP

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

Ready-to-use 150-200 µL

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Cocktail

类型

Antibody

克隆号

2H11+6E1

抗原

Purified human thyroglobulin

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Protein A

保存液

tris with BSA, NP-40

内含物

<0.1% sodium azide

保存条件

4° C

运输条件

Wet ice

产品详细信息

This product is diluted and in a ready-to-use formulation.

A recommended positive control tissue for this product is Thyroid, however positive controls are not limited to this tissue type.

The primary antibody is intended for laboratory professional use in the detection of the corresponding protein in formalin-fixed, paraffin-embedded tissue stained in manual qualitative immunohistochemistry (IHC) testing. This antibody is intended to be used after the primary diagnosis of tumor has been made by conventional histopathology using non-immunological histochemical stains.

Antibody to thyroglobulin has been shown to be useful in positive identification of thyroid carcinomas of the papillary and follicular types. Demonstration of thyroglobulin in a metastatic lesion establishes the thyroid origin of the tumor. Adenocarcinomas of non-thyroidal origin are not reactive. Clone 2H11+6E1 cocktail is especially designed for sensitive detection of thyroglobulin. It stains thyroglobulin in follicular epithelial cells as well as colloid tissue.

Antibody is used with formalin-fixed and paraffin-embedded sections. Pretreatment of deparaffinized tissue with heat-induced epitope retrieval or enzymatic retrieval is recommended. In general, immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody (link antibody), an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.

A positive tissue control must be run with every staining procedure performed. This tissue may contain both positive and negative staining cells or tissue components and serve as both the positive and negative control tissue. External Positive control materials should be fresh autopsy/biopsy/surgical specimens fixed, processed and embedded as soon as possible in the same manner as the patient sample (s). Positive tissue controls are indicative of correctly prepared tissues and proper staining methods. The tissues used for the external positive control materials should be selected from the patient specimens with well-characterized low levels of the positive target activity that gives weak positive staining. The low level of positivity for external positive controls is designed to ensure detection of subtle changes in the primary antibody sensitivity from instability or problems with the staining methodology. A tissue with weak positive staining is more suitable for optimal quality control and for detecting minor levels of reagent degradation.

Internal or external negative control tissue may be used depending on the guidelines and policies that govern the organization to which the end user belongs to. The variety of cell types present in many tissue sections offers internal negative control sites, but this should be verified by the user. The components that do not stain should demonstrate the absence of specific staining, and provide an indication of non-specific background staining. If specific staining occurs in the negative tissue control sites, results with the patient specimens must be considered invalid.

靶标信息

The human thyroglobulin (hTg) is a high molecular weight glycoprotein (605 kDa) found in the thyroid follicular cells. It plays a central role in the uptake, incorporation, and regulated biosynthesis of thyroid hormones, T4 and T3. Thyroid disorders are, in large part, due to autoimmune origin, and anti-thyroglobulin autoantibodies were the first factor to be discovered. Anti-hTG is found in all thyroid autoimmune diseases (Hashimoto thyroiditis, Graves diseases), with the highest level observed in Hashimoto thyroiditis. Anti-hTG is also characteristic of thyroid cancer, and its determination can be used for the follow up of cancer patients.

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