Invitrogen

Tmem119 Monoclonal Antibody (V3RT1GOsz), eBioscience™

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1个已发表图片

2 篇参考文献

查看其他15个TMEM119抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

Tmem119 Antibody in Flow Cytometry (Flow)

图: 1 / 3

Tmem119 Antibody (14-6119-82) in Flow

Demyelinated mouse brain single cell suspension was stained with 0.06 µg Rat IgG2a kappa Isotype Control, purified (Product # 14-4321-82) (left) or 0.06 µg Tmem119 Monoclonal Antibody, purified (right), followed by staining with F(ab')2-Goat anti-Rat IgG (H+L) Secondary Antibody, eFluor 660 (Product # 50-4017-82). Subsequently, cells were stained with CD11b Monoclonal Antibody, PE (Product # 12-0112-82). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).

Product Image Gallery

产品信息

14-6119-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 μg/test

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Not Applicable

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

V3RT1GOsz

抗原

cell surface peptide

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2848273

产品详细信息

Description: This V3RT1GOsz monoclonal antibody recognizes mouse Tmem119. This clone has been reported to be successfully used in flow cytometry.

Applications Reported: This V3RT1GOsz antibody has been reported for use in flow cytometric analysis.

Applications Tested: This V3RT1GOsz antibody has been tested by flow cytometric analysis of mouse brain cells after myelin removal. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

靶标信息

Tmem119 encodes a single-pass type I transmembrane protein which seems to interact with a multitude of micro-RNAs as well as certain transcription factors such as SMAD1, SMAD5 and RUNX2. Tmem119 has been identified as a surface marker of microglia that can be used to reliably distinguish microglia from infiltrating macrophages. Microglial cells are phagocytes that are restricted to the brain and involved in clearing up debris and dendrite pruning of neurons. They display a high functional plasticity, balancing neuroprotective and neurotoxic functions, which is reflected by their distinct physiology in response to changes in their microenvironment. Microglia are particularly mobile in areas of the brain undergoing restructuring. Reactive microglia co-express the marker Iba1. In a pathological context, microglia have been associated with differentiation of pathogenic/inflammatory A1 type astrocytes by secretion of Cq1, IL-1a and TNF when stimulated with LPS, and thought to contribute to the pathology of several neurodegenerative diseases such as Alzheimers, Parkinsons, Chorea Huntingtons disease and Multiple Sclerosis. The specific function Tmem119 plays on microglial cells remains unclear. Outside of the neural system, Tmem119 seems to be involved in osteoblast differentiation/ossification.

仅用于科研。不用于诊断过程。未经明确授权不得转售。