Invitrogen

Transferrin Receptor Monoclonal Antibody (3B82A1)

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

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Transferrin Receptor Antibody in Western Blot (WB)

Transferrin Receptor Antibody in Western Blot (WB)

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Transferrin Receptor Antibody (MA1-40198) in WB

Western blot was performed using Anti-Transferrin Receptor Monoclonal Antibody (3B82A1)(Product # MA1-40198) and a 84kDa band corresponding to Transferrin Receptor was observed across cell line tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), U-87 MG (Lane 3) and MCF-7 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins... View More

Transferrin Receptor Antibody in Western Blot (WB)

Transferrin Receptor Antibody in Western Blot (WB)

Transferrin Receptor Antibody in Western Blot (WB)

Transferrin Receptor Antibody

Transferrin Receptor Antibody

Product Image Gallery

Transferrin Receptor Antibody in Western Blot (WB) — Western blot was performed using Anti-Transferrin Receptor Monoclonal Antibody (3B82A1)(Product # MA1-40198) and a 84kDa band corresponding to Transferrin Receptor was observed across cell line tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), U-87 MG (Lane 3) and MCF-7 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody ( 1:50 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Transferrin Receptor Antibody in Western Blot (WB) — Knockout of Transferrin Receptor was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR944258_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Transferrin Receptor was performed by loading 30 µg of HeLa Wild Type (Lane 1), HeLa Cas9 (Lane 2) andHeLa Transferrin Receptor KO (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with Anti-Transferrin Receptor Monoclonal Antibody (3B82A1) (Product # MA1-40198, 1:50 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:5,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to Transferrin Receptor.

Transferrin Receptor Antibody in Western Blot (WB) — Knockdown of Transferrin Receptor was achieved by transfecting HeLa with Transferrin Receptor specific siRNAs (Silencer® select Product # S725, S726). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Transferrin Receptor knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Transferrin Receptor Monoclonal Antibody (3B82A1) (Product # MA1-40198, 1:50 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Transferrin Receptor.

Transferrin Receptor Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Transferrin Receptor siRNA and decrease in signal intensity was observed in Western Blot application using Anti-Transferrin Receptor Monoclonal Antibody (3B82A1) (Product # MA1-40198). {KD}

Transferrin Receptor Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein inTransferrin Receptor KO cell line compared to control cell line using Anti-Transferrin Receptor Monoclonal Antibody (3B82A1) (Product # MA1-40198). {KO}

产品信息

MA1-40198

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:50

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免疫组化（冰冻） (IHC (F))

1:50

-

流式细胞分析 (Flow)

1:50

-

酶联免疫吸附实验 (ELISA)

1:50

-

免疫沉淀 (IP)

1:50

-

抑制试剂 (IA)

-

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

3B82A1

抗原

Human Transferrin receptor 1.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Protein G

保存液

PBS with 0.1% BSA

内含物

0.02% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2287187

产品详细信息

MA1-40198 detects Transferrin receptor 1 from human samples.

MA1-40198 has been successfully used in flow cytometry, ELISA, immunohistochemistry (frozen), immunoprecipitation, and Western blot applications.

The MA1-40198 immunogen is human Transferrin receptor 1.

靶标信息

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Application of In vitro transcytosis models to brain targeted biologics.

PloS one

Deng K,Lu Y,Finnema SJ,Vangjeli K,Huang J,Huang L,Goodearl A

MA1-40198 was used in Inhibition Assays to evaluate human models including human cerebral microvascular endothelial cell line hCMEC/D3 and human colon epithelial cell line Caco-2 models.

Fri Aug 25 00:00:00 EDT 2023

Characterisation of hepcidin response to holotransferrin treatment in CHO TRVb-1 cells.

Blood cells, molecules & diseases

Mehta K,Greenwell P,Renshaw D,Busbridge M,Garcia M,Farnaud S,Patel VB

MA1-40198 was used in western blot to elucidate the role of transferrin receptor and intracellular iron in hepcidin secretion.

Sat Aug 01 00:00:00 EDT 2015

生物信息学

蛋白别名: CD antigen CD71; CD71; p90; T9; TR; Transferrin receptor protein 1

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基因别名: CD71; IMD46; p90; T9; TFR; TFR1; TFRC; TR; TRFR

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UniProt ID: (Human) P02786

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Entrez Gene ID: (Human) 7037

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virus receptor activity glycoprotein binding double-stranded RNA binding transferrin receptor activity protein binding Hsp70 protein binding transferrin transmembrane transporter activity identical protein binding protein homodimerization activity poly(A) RNA binding chaperone binding metalloprotease

参与通路

regulation of cell growth response to hypoxia cellular iron ion homeostasis acute-phase response aging response to nutrient response to iron ion response to manganese ion osteoclast differentiation positive regulation of B cell proliferation receptor internalization response to retinoic acid transferrin transport cellular response to drug positive regulation of T cell proliferation regulation of cell proliferation positive regulation of bone resorption positive regulation of isotype switching response to copper ion viral entry into host cell iron ion import