Invitrogen

TrxR1 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (9)
高级验证 (1)

TrxR1 Antibody in Immunocytochemistry (ICC/IF)

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TrxR1 Antibody (PA5-28886) in ICC/IF

Immunofluorescence analysis of TrxR1 was performed using 70 confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TrxR1 Polyclonal Antibody (Product # PA5-28886) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A3279... View More

TrxR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TrxR1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A549 cells were transfected with TrxR1 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-TrxR1 Polyclonal Antibody (Product # PA5-28886). {KD}

产品信息

PA5-28886

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 231 and 488 of Human TrxR1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2546362

产品详细信息

Recommended positive controls: Molt-4, mouse brain, rat brain.

Predicted reactivity: Mouse (93%), Rat (91%), Dog (96%), Pig (93%), Rabbit (96%), Chicken (85%), Rhesus Monkey (98%), Chimpanzee (99%), Bovine (93%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: gene associated with retinoic and IFN-induced mortality 12 protein; Gene associated with retinoic and interferon-induced mortality 12 protein; GRIM-12; KM-102-derived reductase-like factor; MGC9145; NADPH-dependent thioredoxin reductase; oxidoreductase; Peroxidase TXNRD1; selenoprotein oxidoreductase; testis tissue sperm-binding protein Li 46a; Thioredoxin reductase 1, cytoplasmic; thioredoxin reductase GRIM-12; Thioredoxin reductase TR1; TR; TR alpha

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基因别名: GRIM-12; GRIM12; KDRF; TR; TR1; TRXR1; TXNR; TXNRD1

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UniProt ID: (Human) Q16881, (Mouse) Q9JMH6

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Entrez Gene ID: (Human) 7296, (Rat) 58819, (Mouse) 50493

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