Invitrogen

Tyrosinase Monoclonal Antibody (T311)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

6个已发表图片

10 篇参考文献

查看其他33个Tyrosinase抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

Tyrosinase Antibody in Western Blot (WB)

图: 1 / 9

Tyrosinase Antibody (35-6000) in WB

Western blot was performed using Anti-Tyrosinase Monoclonal Antibody (T311) (Product # 35-6000) and an 80 kDa band corresponding to Tyrosinase was observed across all the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of A-375 (Lane 1), A-431 (Lane 2), SK-MEL-31 (Lane 3), SK-MEL-5 (Lane 4), B16-F10 (Lane 5), HaCaT (Lane 6), Mouse Retina (Lane 7), Mouse Lung (Lane 8) or Mouse Spleen (Lane 9) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Ni... View More

Tyrosinase Antibody in Immunohistochemistry (IHC)

Tyrosinase Antibody in Flow Cytometry (Flow)

Product Image Gallery

产品信息

35-6000

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:250

查看5篇已发表文章 5篇已发表文章

免疫组化 (IHC)

Assay-dependent

查看4篇已发表文章 4篇已发表文章

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Rat

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

T311

抗原

Recombinant tyrosinase protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533214

靶标信息

The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Chemical Constituents from Streblus taxoides Wood with Their Antibacterial and Antityrosinase Activities Plus in Silico Study.

Antibiotics (Basel, Switzerland)

Parndaeng K,Pitakbut T,Wattanapiromsakul C,Hwang JS,Udomuksorn W,Dej-Adisai S

Published figure using Tyrosinase monoclonal antibody (Product # 35-6000) in Western Blot

Fri Feb 03 00:00:00 EST 2023

Mechanism of action of 4-substituted phenols to induce vitiligo and antimelanoma immunity.

Pigment cell & melanoma research

Kammeyer A,Willemsen KJ,Ouwerkerk W,Bakker WJ,Ratsma D,Pronk SD,Smit NPM,Luiten RM

35-6000 was used in Western Blotting to study the influence of the chemical structure on the biological activity of 4 substituted phenols.

Mon Jul 01 00:00:00 EDT 2019

Mechanism of action of 4-substituted phenols to induce vitiligo and antimelanoma immunity.

Pigment cell & melanoma research

Kammeyer A,Willemsen KJ,Ouwerkerk W,Bakker WJ,Ratsma D,Pronk SD,Smit NPM,Luiten RM

35-6000 was used in Western Blotting to study the influence of the chemical structure on the biological activity of 4 substituted phenols.

Mon Jul 01 00:00:00 EDT 2019

Absence of recognition of common melanocytic antigens by T cells isolated from the cerebrospinal fluid of a Vogt-Koyanagi-Harada patient.

Molecular vision

Abad S,Wieërs G,Colau D,Wildmann C,Delair E,Dhote R,Brézin AP,Kawakami Y,Coulie PG,van der Bruggen P

35-6000 was used in western blot to identify antigens recognized by autoreactive CD4 T lymphocytes isolated from a Vogt-Koyanagi-Harada patient who did not express HLA-DRB1*04:05

Tue Sep 30 00:00:00 EDT 2014

Cultivation-dependent plasticity of melanoma phenotype.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Kodet O,Dvořánková B,Krejčí E,Szabo P,Dvořák P,Štork J,Krajsová I,Dundr P,Smetana K,Lacina L

Sun Dec 01 00:00:00 EST 2013

Flow cytometry assessment of residual melanoma cells in tumor-infiltrating lymphocyte cultures.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Richards JO,Treisman J,Garlie N,Hanson JP,Oaks MK

Tue May 01 00:00:00 EDT 2012

Flow cytometry assessment of residual melanoma cells in tumor-infiltrating lymphocyte cultures.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Richards JO,Treisman J,Garlie N,Hanson JP,Oaks MK

Tue May 01 00:00:00 EDT 2012

Flow cytometry assessment of residual melanoma cells in tumor-infiltrating lymphocyte cultures.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Richards JO,Treisman J,Garlie N,Hanson JP,Oaks MK

Tue May 01 00:00:00 EDT 2012

T-cell immune function in tumor, skin, and peripheral blood of advanced stage melanoma patients: implications for immunotherapy.

Clinical cancer research : an official journal of the American Association for Cancer Research

Tjin EP,Konijnenberg D,Krebbers G,Mallo H,Drijfhout JW,Franken KL,van der Horst CM,Bos JD,Nieweg OE,Kroon BB,Haanen JB,Melief CJ,Vyth-Dreese FA,Luiten RM

Thu Sep 01 00:00:00 EDT 2011

Mitochondrial proteomics of the retinal pigment epithelium at progressive stages of age-related macular degeneration.

Investigative ophthalmology & visual science

Nordgaard CL,Karunadharma PP,Feng X,Olsen TW,Ferrington DA

35-6000 was used in western blot to examine the subproteome of mitochondria isolated from human donor retinal pigment epithelium graded with the Minnesota Grading System