Invitrogen

Tyrosine Hydroxylase Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

11个已发表图片

11 篇参考文献

查看其他34个Tyrosine Hydroxylase抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

Tyrosine Hydroxylase Antibody in Immunocytochemistry (ICC/IF)

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Tyrosine Hydroxylase Antibody (PA1-4679) in ICC/IF

Immunofluorescence analysis of Anti-Tyrosine Hydroxylase Polyclonal Antibody (Product # PA1-4679) was performed using 70% confluent log phase PC-12 cells treated with 5 µg/mL dexamethasone for 6 days. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Anti-... View More

Tyrosine Hydroxylase Antibody in Immunohistochemistry (IHC)

Tyrosine Hydroxylase Antibody in Western Blot (WB)

Tyrosine Hydroxylase Antibody in Immunohistochemistry (Frozen) (IHC (F))

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产品信息

PA1-4679

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

1:1,000

查看7篇已发表文章 7篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:1,000

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Many, Mouse, Rat

已发表种属

Dog, Human, Mouse, Rat

宿主/亚型

Sheep / IgG

分类

Polyclonal

类型

Antibody

抗原

SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

~0.15 mg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_561880

产品详细信息

This antibody is specific for the ~60 kDa Tyrosine Hydroxylase in Western blots of rat brain extracts.

靶标信息

Tyrosine hydroxylase (TH) is an enzyme involved in the synthesis of catecholamine neurotransmitters dopamine, epinephrine, and norepinephrine. In all species, catecholamine synthesis is regulated by the interaction of TH with a cofactor, tetrahydrobiopterin (BH4). BH4 binds to the TH catalytic domain, resulting in enzymatic activity. Unlike TH in non-primate species, four human TH mRNA splice variants (hTH1-hTH4) have been isolated. These variants are identical in their catalytic domain, but differ in their N-terminal, regulatory domains. TH is also responsible for the conversion of L-tyrosine to L-dopa. TH plays a key role in the physiology of adrenergic neurons. The role of TH in the synthesis of catecholamine neurotransmitters suggests a correlation between the enzyme and a number of neuropathogenic diseases including: Parkinson's disease, schizophrenia, Segawa syndrome, and dystonia, as well as a variety of cardiovascular diseases.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Striatonigral distribution of a fluorescent reporter following intracerebral delivery of genome editors.

Frontiers in bioengineering and biotechnology

Neuman SS,Metzger JM,Bondarenko V,Wang Y,Felton J,Levine JE,Saha K,Gong S,Emborg ME

PA1-4679 was used in Immunohistochemistry to report the effect of injecting genome editors into the striatum of Ai14 reporter mice, which have a LoxP-flanked stop cassette that prevents expression of the red fluorescent protein tdTomato.

Sat Aug 12 00:00:00 EDT 2023

Methanolic Extract of Boswellia serrata Gum Protects the Nigral Dopaminergic Neurons from Rotenone-Induced Neurotoxicity.

Molecular neurobiology

Shadfar S,Khanal S,Bohara G,Kim G,Sadigh-Eteghad S,Ghavami S,Choi H,Choi DY

PA1-4679 was used in Immunohistochemistry to suggest that Boswellia protected the dopaminergic neurons from rotenone neurotoxicity via activation of the AMPK pathway which might be associated with attenuation of α-synuclein aggregation and neuroinflammation.

Thu Sep 01 00:00:00 EDT 2022

Post-Ganglionic Sympathetic Neurons can Directly Sense Raised Extracellular Na⁺ via SCN7a/Na_x.

Frontiers in physiology

Davis H,Paterson DJ,Herring N

Published figure using Tyrosine Hydroxylase polyclonal antibody (Product # PA1-4679) in Immunocytochemistry

Sun Sep 24 00:00:00 EDT 2023

DJ-1 is an essential downstream mediator in PINK1/parkin-dependent mitophagy.

Brain : a journal of neurology

Imberechts D,Kinnart I,Wauters F,Terbeek J,Manders L,Wierda K,Eggermont K,Madeiro RF,Sue C,Verfaillie C,Vandenberghe W

PA1-4679 was used in Immunocytochemistry to suggest that disruption of PINK1/parkin/DJ-1-mediated mitophagy is a common pathogenic mechanism in autosomal recessive Parkinson's disease.

Mon Dec 19 00:00:00 EST 2022

Activation of the Lateral Habenula-Ventral Tegmental Area Neural Circuit Contributes to Postoperative Cognitive Dysfunction in Mice.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Xin J,Shan W,Li J,Yu H,Zuo Z

PA1-4679 was used in Immunohistochemistry-immunofluorescence to suggest that surgery activates the LHb-VTA neural circuit, which contributes to POCD and neuropathological changes in the brain.

Mon Aug 01 00:00:00 EDT 2022

The Mesolimbic Dopamine Activity Signatures of Relapse to Alcohol-Seeking.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Liu Y,Jean-Richard-Dit-Bressel P,Yau JO,Willing A,Prasad AA,Power JM,Killcross S,Clifford CWG,McNally GP

PA1-4679 was used in Immunohistochemistry to show how different forms of relapse to alcohol-seeking in male rats are assembled from activity across the VTA and the nucleus accumbens.

Wed Aug 12 00:00:00 EDT 2020

Parkin deficiency accelerates consequences of mitochondrial DNA deletions and Parkinsonism.

Neurobiology of disease

Song L,McMackin M,Nguyen A,Cortopassi G

PA14679 was used in immunohistochemistry - frozen section and western blot to assess the effect of increased Parkin deletion mutagenesis in the absence of mitochondrial quality control

Sat Apr 01 00:00:00 EDT 2017

Loss of the transcription factor Meis1 prevents sympathetic neurons target-field innervation and increases susceptibility to sudden cardiac death.

eLife

Bouilloux F,Thireau J,Ventéo S,Farah C,Karam S,Dauvilliers Y,Valmier J,Copeland NG,Jenkins NA,Richard S,Marmigère F

PA1-4679 was used in immunohistochemistry - frozen section to determine how the loss of a transcription factor, Meis1, prevents innervation of sympathetic neurons and increases the likelihood of sudden cardiac death

Mon Feb 08 00:00:00 EST 2016

Abnormal sinoatrial node development resulting from disturbed vascular endothelial growth factor signaling.

International journal of cardiology

Calkoen EE,Vicente-Steijn R,Hahurij ND,van Munsteren CJ,Roest AA,DeRuiter MC,Steendijk P,Schalij MJ,Gittenberger-de Groot AC,Blom NA,Jongbloed MR

PA1-4679 was used in immunohistochemistry to study the role of vascular endothelial growth factor in sinoatrial node development.

Sun Mar 15 00:00:00 EDT 2015

Astroglial heme oxygenase-1 and the origin of corpora amylacea in aging and degenerating neural tissues.

Experimental neurology

Song W,Zukor H,Liberman A,Kaduri S,Arvanitakis Z,Bennett DA,Schipper HM

PA1-4679 was used in immunohistochemistry to study the role of astroglial heme oxygenase-1 in the generation of corpora amylacea in cultured cells and in the aging brain and the therapeutic implications for neurodegenerative diseases

Tue Apr 01 00:00:00 EDT 2014

Catheter-based renal sympathetic denervation significantly inhibits atrial fibrillation induced by electrical stimulation of the left stellate ganglion and rapid atrial pacing.

PloS one

Hou Y,Hu J,Po SS,Wang H,Zhang L,Zhang F,Wang K,Zhou Q

PA1-4679 was used in immunohistochemistry to study the effects of renal sympathetic denervation on atrial fibrillation in a canine hyper-sympathetic tone model

Fri Aug 08 00:00:00 EDT 2014

生物信息学

蛋白别名: dystonia 14; EC 1.14.16.2; HGNC:11782; TH; TH isoform 3; TH isoform a; TH-4; TY3H; TYH antibody; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase

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基因别名: DYT14; DYT5b; TH; The; TYH

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UniProt ID: (Human) P07101, (Mouse) P24529, (Rat) P04177

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Entrez Gene ID: (Human) 7054, (Mouse) 21823, (Rat) 25085

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