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UBA3 Antibody (CF811882) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBA3 (RC204675, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-UBA3. (1. Positive lysates LY418315 (100 µg) and LC418315 (20 µg) can be purchased separately from OriGene.
产品信息
CF811882
产品规格
种属反应
Dog,
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
OTI3F9
抗原
Human recombinant protein fragment corresponding to amino acids 1-463 of human UBA3 produced in E.coli.
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 8% trehalose
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
靶标信息
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DKFZp566J164; MGC22384; NEDD8-activating enzyme E1 catalytic subunit; NEDD8-activating enzyme E1 subunit 2; NEDD8-activating enzyme E1C; Nedd8-activating enzyme hUba3; UBA3, ubiquitin-activating enzyme E1 homolog; Ubiquitin-activating enzyme 3; Ubiquitin-activating enzyme E1C; ubiquitin-activating enzyme E1C (homologous to yeast UBA3); ubiquitin-activating enzyme E1C (UBA3 homolog, yeast); ubiquitin-like modifier activating enzyme 3; Ubiquitin-like modifier-activating enzyme 3
基因别名: A830034N06Rik; AI256736; AI848246; AW546539; hUBA3; NAE2; UBA3; UBE1C
UniProt ID: (Human) Q8TBC4, (Mouse) Q8C878, (Rat) Q99MI7
Entrez Gene ID: (Human) 9039, (Dog) 476560, (Mouse) 22200, (Rat) 117553
