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UBE2D3 Antibody (PA5-119881) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of UBE2D3 in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with UBE2D3 Polyclonal Antibody (Product # PA5-119881) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).
产品信息
PA5-119881
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant full length protein of human UBE2D3
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2913453
产品详细信息
Positive Control: Jurkat cell lysate, 293T cell lysate, MCF-7 cell lysate, rat liver tissue lysate, mouse lung tissue lysate, SW620, mouse brain tissue.
Subcellular Location: Cell membrane, Endosome membrane.
靶标信息
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: (E3-independent) E2 ubiquitin-conjugating enzyme D3; E2 ubiquitin-conjugating enzyme D3; E2(17)KB 3; MGC43926; MGC5416; PAPase; Phosphoarginine phosphatase; PRO2116; Ubiquitin carrier protein D3; ubiquitin conjugating enzyme E2D 3; Ubiquitin-conjugating enzyme E2 D3; Ubiquitin-conjugating enzyme E2(17)KB 3; Ubiquitin-conjugating enzyme E2-17 kDa 3; ubiquitin-conjugating enzyme E2D 3 (homologous to yeast UBC4/5); ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase D3
基因别名: 1100001F19Rik; 9430029A22Rik; AA414951; E2(17)KB3; UBC4/5; UBC5C; UBCH5C; UBE2D3
UniProt ID: (Human) P61077, (Mouse) P61079, (Rat) P61078
Entrez Gene ID: (Human) 7323, (Mouse) 66105, (Rat) 81920
negative regulation of transcription from RNA polymerase II promoter
protein polyubiquitination
DNA repair
cellular protein modification process
ubiquitin-dependent protein catabolic process
protein monoubiquitination
apoptotic process
protein ubiquitination
BMP signaling pathway
TRIF-dependent toll-like receptor signaling pathway
proteasome-mediated ubiquitin-dependent protein catabolic process
protein autoubiquitination
regulation of transcription from RNA polymerase II promoter in response to hypoxia
protein K48-linked ubiquitination
protein K11-linked ubiquitination
positive regulation of protein targeting to mitochondrion
cellular response to DNA damage stimulus
