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For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates.The first step requires the ATP-dependent activation of the Ub C-terminusand the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. A wide range of enzymes facilitate in the proteolytic Ub pathway including UBE2E3, also designated UBCH9, which catalyzes the covalent attachment of ubiquitin to other proteins and is involved in the regulation of transepithelial sodium transport in renal cells. UBE2E3 may also be involved in cell growth arrest. The UBE2E3 protein shuttles between the cytoplasm and nucleus in a IPO11-dependent manner. It is ubiquitously expressed at low levels and is highly expressed in skeletal muscle.
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蛋白别名: E2 ubiquitin-conjugating enzyme E3; UbcH9; UbcM2; Ubiquitin carrier protein E3; ubiquitin conjugating enzyme E2E 3; ubiquitin-conjugating enzyme 4; Ubiquitin-conjugating enzyme E2 E3; Ubiquitin-conjugating enzyme E2-23 kDa; ubiquitin-conjugating enzyme E2E 3 (homologous to yeast UBC4/5); ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme E2E 3, UBC4/5 homolog; Ubiquitin-protein ligase E3
基因别名: UBCE4; UBCH9; UbcM2; UBE2E3
UniProt ID: (Human) Q7Z4W4, (Mouse) P52483
Entrez Gene ID: (Dog) 612823, (Human) 10477, (Mouse) 22193