Invitrogen

UFC1 Recombinant Rabbit Monoclonal Antibody (JE65-12)

查看其他8个UFC1抗体

抗体检测数据 (4)

UFC1 Antibody in Immunocytochemistry (ICC/IF)

UFC1 Antibody in Immunocytochemistry (ICC/IF)

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UFC1 Antibody (MA5-44697) in ICC/IF

Immunocytochemistry analysis of UFC1 in SH-SY5Y cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 , permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Samples were incubated in UFC1 Monoclonal antibody (Product # MA5-44697) using a dilution of 1:400 in 2% negative goat serum overnight at 4 followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at a dilution of 1:1,000. Nuclear DNA was labelled in blue with DAPI.

UFC1 Antibody in Immunocytochemistry (ICC/IF)

UFC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

UFC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

UFC1 Antibody in Western Blot (WB)

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UFC1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of UFC1 in SH-SY5Y cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 , permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Samples were incubated in UFC1 Monoclonal antibody (Product # MA5-44697) using a dilution of 1:400 in 2% negative goat serum overnight at 4 followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at a dilution of 1:1,000. Nuclear DNA was labelled in blue with DAPI.

UFC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of UFC1 in paraffin-embedded human breast carcinoma tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with UFC1 Monoclonal antibody (Product # MA5-44697) using a dilution of 1:400 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

UFC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of UFC1 in paraffin-embedded human trachea tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with UFC1 Monoclonal antibody (Product # MA5-44697) using a dilution of 1:400 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

UFC1 Antibody in Western Blot (WB) — Western blot analysis of UFC1 in 293T cell lysates (10 µg/Lane). Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Samples were incubated in UFC1 Monoclonal antibody (Product # MA5-44697) using a dilution of 1:1,000 in 5% NFDM/TBST at room temperature for 2 hours followed by Goat Anti-Rabbit IgG - HRP secondary antibody at a dilution of 1:300,000 for 1 hour at room temperature. Predicted band size: 19 kDa. Observed band size: 19 Da.

产品信息

MA5-44697

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

-

免疫组化（石蜡） (IHC (P))

1:400

-

免疫细胞化学 (ICC/IF)

1:400

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JE65-12

抗原

Recombinant protein within human UFC1 aa 1-150/167.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2931155

靶标信息

UFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1 (Komatsu et al., 2004 ).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Ubiquitin-fold modifier-conjugating enzyme 1; Ufm1-conjugating enzyme 1

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基因别名: CGI-126; HSPC155; UFC1

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UniProt ID: (Human) Q9Y3C8

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Entrez Gene ID: (Human) 51506

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protein binding UFM1 transferase activity

参与通路

response to endoplasmic reticulum stress protein ufmylation protein K69-linked ufmylation