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ULK1 Antibody (PA5-85733) in WB
Western Blot analysis of ULK1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ULK1 Polyclonal Antibody (Product # PA5-85733) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
产品信息
PA5-85733
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ULK1.
偶联物
形式
Liquid
浓度
0.24 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol, 1% BSA
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2792872
产品详细信息
Keep as concentrated solution.
Predicted reactivity: Mouse (100%).
Positive Control: Jurkat, IMR32, SK-N-AS.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: APG1; ATG 1; ATG1; ATG1 autophagy related 1 homolog; Autophagy-related protein 1 homolog; C. elegans; EC:2.7.11.1; FLJ38455; Serine/threonine-protein kinase ULK1; ULK 1; UNC 51; Unc-51-like kinase 1
基因别名: ATG1; ATG1A; hATG1; KIAA0722; ULK1; UNC51; Unc51.1
UniProt ID: (Human) O75385
Entrez Gene ID: (Human) 8408
protein phosphorylation
signal transduction
protein localization
regulation of autophagy
positive regulation of autophagy
macroautophagy
positive regulation of macroautophagy
peptidyl-serine phosphorylation
neuron projection regeneration
neuron projection development
negative regulation of protein complex assembly
cellular response to nutrient levels
response to starvation
protein autophosphorylation
axon extension
