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Invitrogen
Ubiquitin Monoclonal Antibody (2A1)
高级验证
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
图: 1 / 4
Ubiquitin Antibody (LF-MA0118) in ICC/IF
Immunofluorescence analysis of Ubiquitin B was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ubiquitin (2A1) Mouse Monoclonal Antibody at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room te... View More
产品信息
LF-MA0118
应用
建议稀释比
已发表文章
产品规格
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
2A1
抗原
Purified, recombinant, human ubiquitin GST-fusion protein expressed in E. coli.
偶联物
形式
Liquid
浓度
1.0 mg/mL
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_1088151
产品详细信息
A suggested positive control for this product is Bosc23 cells transfected with GFP-Ub.
靶标信息
Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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