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Uricase Antibody (200-103-092-0100) in WB
Western Blot of Goat anti-Uricase Peroxidase Conjugated antibody. Lane 1: purified Uricase under reducing conditions. Lane 2: purified Uricase under reducing conditions. Lane 3: purified Uricase under non-reducing conditions. Lane 4: purified Uricase under non-reducing conditions Load: ~1.0 µg (Lane 1 and 3) and 0.25 µg (Lane 2 and 4). Primary antibody: Uricase Peroxidase Conjugated Antibody at 1:5,000 overnight at 4°C. Secondary antibody: DyLight™ 649 goat secondary antibody at 1:10,000 for 90 min at RT. Block: 5% BLOTTO overnight at 4... View More
产品信息
200-103-092-0100
应用
建议稀释比
已发表文章
产品规格
种属反应
Bacteria
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Anti-uricase Antibody was produced by repeated immunizations with bacillus uricase protein.
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Ion-exchange chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA
内含物
0.01% gentamicin sulfate
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
产品详细信息
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Uricase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Uricase [Bacillus species]. Cross reactivity against Uricase from other sources is unknown.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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