Invitrogen

V5 Tag Monoclonal Antibody, Alexa Fluor™ 647

产品信息

451098

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

查看3篇已发表文章 3篇已发表文章

酶联免疫吸附实验 (ELISA)

查看2篇已发表文章 2篇已发表文章

抑制试剂 (IA)

Assay-dependent

其他PubMed (Misc)

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产品规格

种属反应

Tag

已发表种属

Tag

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

抗原

sequence -Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-

偶联物

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

激发/发射光谱

650/671 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

purified

保存液

PBS, pH 7.5

内含物

2mM sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2532221

产品详细信息

The Alexa FluorTM dyes provide for extraordinarily bright antibody conjugates. At the time of preparation, the products are certified to be free of unconjugated dyes and are tested in a cytological experiment to ensure low nonspecific staining. For Alexa FluorTM 647 the approximate fluorescence excitation (Ex) and emission (Em) maxima, in nm: Ex 650/Em 668.

靶标信息

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

An AKT2-specific nanobody that targets the hydrophobic motif induces cell cycle arrest, autophagy and loss of focal adhesions in MDA-MB-231 cells.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Merckaert T,Zwaenepoel O,Gevaert K,Gettemans J

451098 was used in Flow Cytometry to investigate the role of AKT2 in the MDA-MB-231 breast cancer cell model.

Fri Jan 01 00:00:00 EST 2021

NIK promotes metabolic adaptation of glioblastoma cells to bioenergetic stress.

Cell death & disease

Kamradt ML,Jung JU,Pflug KM,Lee DW,Fanniel V,Sitcheran R

451098 was used in Flow cytometry/Cell sorting to show that NIK is recruited to the outer membrane of dividing mitochondria with the master fission regulator, Dynamin-related protein1 (DRP1).

Mon Mar 15 00:00:00 EDT 2021

Replication Study: Melanoma genome sequencing reveals frequent PREX2 mutations.

eLife

Horrigan SK,Courville P,Sampey D,Zhou F,Cai S

451098 was used in western blot to find frequent phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 mutations via melanoma genome sequencing

Thu Jan 19 00:00:00 EST 2017

Distinct oligoclonal band antibodies in multiple sclerosis recognize ubiquitous self-proteins.

Proceedings of the National Academy of Sciences of the United States of America

Brändle SM,Obermeier B,Senel M,Bruder J,Mentele R,Khademi M,Olsson T,Tumani H,Kristoferitsch W,Lottspeich F,Wekerle H,Hohlfeld R,Dornmair K

451098 was used to analyze the recognition of ubiquitous self-proteins by distinct oligoclonal band antibodies in multiple sclerosis

Tue Jul 12 00:00:00 EDT 2016

High-throughput allogeneic antibody detection using protein microarrays.

Journal of immunological methods

Paul J,Sahaf B,Perloff S,Schoenrock K,Wu F,Nakasone H,Coller J,Miklos D

451098 was used in ELISA to study a high-throughput method using protein microarrays to detect allogeneic antibodies

Sun May 01 00:00:00 EDT 2016

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions.

Journal of visualized experiments : JoVE

Fasolo J,Im H,Snyder MP

451098 was used in ELISA to study kinase protein-protein interactions.

Sun Aug 02 00:00:00 EDT 2015

Estimating the fitness advantage conferred by permissive neuraminidase mutations in recent oseltamivir-resistant A(H1N1)pdm09 influenza viruses.

PLoS pathogens

Butler J,Hooper KA,Petrie S,Lee R,Maurer-Stroh S,Reh L,Guarnaccia T,Baas C,Xue L,Vitesnik S,Leang SK,McVernon J,Kelso A,Barr IG,McCaw JM,Bloom JD,Hurt AC

451098 was used in flow cytometry to discover the advantage of neuraminidase mutations in oseltamivir-resistant A(H1N1)pdm09 influenza viruses

Tue Apr 01 00:00:00 EDT 2014

生物信息学

蛋白别名: simian virus 5 antibody; SPV5gp2; sv5; v-5; V5; V5 Epitope Tag

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