Invitrogen

V5 Tag Monoclonal Antibody, AP

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V5 Tag Antibody (R962-25) in WB

Western blot analysis of V5 tag was performed by loading 20 µg of whole cell extracts of untransfected HEK-293 and HEK-293 cells transiently overexpressing V5-His- LacZ using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), and Novex® Sharp Pre-Stained Protein Standard (LC5800). Proteins were transferred to a PVDF membrane and blocked with 5% skim milk for 1 hour at room temperature. V5-His- LacZ was detected at ~117 kDa using V5-AP Mouse Monoclonal Antibody (Product # R962... View More

V5 Tag Antibody in Immunoprecipitation (IP)

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产品信息

R962-25

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

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免疫沉淀 (IP)

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其他PubMed (Misc)

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产品规格

种属反应

Tag

已发表种属

Tag

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

抗原

V5 synthetic peptide: Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-.

偶联物

AP AP AP

形式

Liquid

浓度

1-2 mg/mL

纯化类型

purified

保存液

PBS

内含物

0.01% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2556566

产品详细信息

NOTE: R962-25 was previously sold under product #46-0287. When re-ordering, please use #R962-25.

R962-25 recognizes amino acid sequence: -Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr-.

This product contains enough material for 25 Western blots. Western blot experiments with 100 ng of recombinant Positope™ control protein gave a detectable signal within 15 minutes using a chromogenic substrate. The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His(C-term), HisG, c-myc, and V5. For Western blot, we recommend diluting the AP-conjugated antibody in Tris-Buffered Saline (TBS) containing 0.1% (v/v) Tween-20 and 1% (w/v) nonfat dry milk.

In Western blot applications, no cross-reactivity has been observed in bacterial lysates.

靶标信息

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Humans with inherited T cell CD28 deficiency are susceptible to skin papillomaviruses but are otherwise healthy.

Cell

Béziat V,Rapaport F,Hu J,Titeux M,Bonnet des Claustres M,Bourgey M,Griffin H,Bandet É,Ma CS,Sherkat R,Rokni-Zadeh H,Louis DM,Changi-Ashtiani M,Delmonte OM,Fukushima T,Habib T,Guennoun A,Khan T,Bender N,Rahman M,About F,Yang R,Rao G,Rouzaud C,Li J,Shearer D,Balogh K,Al Ali F,Ata M,Dabiri S,Momenilandi M,Nammour J,Alyanakian MA,Leruez-Ville M,Guenat D,Materna M,Marcot L,Vladikine N,Soret C,Vahidnezhad H,Youssefian L,Saeidian AH,Uitto J,Catherinot É,Navabi SS,Zarhrate M,Woodley DT,Jeljeli M,Abraham T,Belkaya S,Lorenzo L,Rosain J,Bayat M,Lanternier F,Lortholary O,Zakavi F,Gros P,Orth G,Abel L,Prétet JL,Fraitag S,Jouanguy E,Davis MM,Tangye SG,Notarangelo LD,Marr N,Waterboer T,Langlais D,Doorbar J,Hovnanian A,Christensen N,Bossuyt X,Shahrooei M,Casanova JL

R962-25 was used in Western Blotting to conclude that human CD28-dependent T cell responses are largely redundant for protective immunity.

Thu Jul 08 00:00:00 EDT 2021

DNA polymerase zeta contributes to heterochromatin replication to prevent genome instability.

The EMBO journal

Ben Yamin B,Ahmed-Seghir S,Tomida J,Despras E,Pouvelle C,Yurchenko A,Goulas J,Corre R,Delacour Q,Droin N,Dessen P,Goidin D,Lange SS,Bhetawal S,Mitjavila-Garcia MT,Baldacci G,Nikolaev S,Cadoret JC,Wood RD,Kannouche PL

Published figure using V5 Tag monoclonal antibody (Product # R962-25) in Immunoprecipitation

Tue Nov 02 00:00:00 EDT 2021

Dual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations.

The Journal of experimental medicine

Wang Y,Ma CS,Ling Y,Bousfiha A,Camcioglu Y,Jacquot S,Payne K,Crestani E,Roncagalli R,Belkadi A,Kerner G,Lorenzo L,Deswarte C,Chrabieh M,Patin E,Vincent QB,Müller-Fleckenstein I,Fleckenstein B,Ailal F,Quintana-Murci L,Fraitag S,Alyanakian MA,Leruez-Ville M,Picard C,Puel A,Bustamante J,Boisson-Dupuis S,Malissen M,Malissen B,Abel L,Hovnanian A,Notarangelo LD,Jouanguy E,Tangye SG,Béziat V,Casanova JL

R96225 was used in western blot to identify RLTPR in patients and determine the effects of these mutations on CD4 positive T cells

Mon Oct 17 00:00:00 EDT 2016

Conserved overlapping gene arrangement, restricted expression, and biochemical activities of DNA polymerase ν (POLN).

The Journal of biological chemistry

Takata K,Tomida J,Reh S,Swanhart LM,Takata M,Hukriede NA,Wood RD

R962-25 was used in immunoprecipitation to study the expression, biological roles, and biochemical activities of DNA polymerase v

Fri Oct 02 00:00:00 EDT 2015

Over-expression of the BRMS1 family member SUDS3 does not suppress metastasis of human cancer cells.

Cancer letters

Silveira AC,Hurst DR,Vaidya KS,Ayer DE,Welch DR

R962-25 was used in Western Blotting to demonstrate functional differences in BRMS1 family members, and highlight the differences in SIN3-HDAC composition affecting protein expression and biological behaviours.

Wed Apr 08 00:00:00 EDT 2009

生物信息学

蛋白别名: simian virus 5 antibody; SPV5gp2; sv5; v-5; V5; V5 Epitope Tag

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