Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 3
VAChT Antibody (MA5-45502) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with VAChT monoclonal antibody (Product # MA5-45502) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min at RT, 5 min at RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin st... View More
产品信息
MA5-45502
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
N6/38
抗原
Synthetic peptide amino acids 521-532 of human VAChT
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
47.35mM phosphate/2.15mM MES, pH 7.4, with 2mM EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2931956
产品详细信息
A dilution of 1:50-1:200 of MA5-45502 was sufficient for detection of VAChT Transporter in rat brain using immunohistochemistry analysis and goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 56kDa.
This antibody was formerly sold as clone S6-38.
靶标信息
This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: MGC12716; solute carrier family 18 (vesicular acetylcholine transporter), member 3; solute carrier family 18 (vesicular acetylcholine), member 3; solute carrier family 18 (vesicular monoamine) member 3; Solute carrier family 18 member 3; solute carrier family 18, member 3; VAChT; Vesicular acetylcholine transporter
基因别名: rVAT; SLC18A3; VACHT; VAT
UniProt ID: (Human) B2R7S1, (Rat) Q62666
Entrez Gene ID: (Human) 6572, (Mouse) 20508, (Rat) 60422
