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VAChT Antibody (MA5-27662) in ICC/IF
Immunofluorescent analysis of VAChT in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with VAChT monoclonal antibody (Product # MA5-27662) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) VAChT Antibody, D) Merged image. Magnification... View More
产品信息
MA5-27662
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
N6/38
抗原
Synthetic peptide amino acids 521-532 of human VAChT
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.09% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2735407
产品详细信息
A dilution of 1:50-1:200 of MA5-27662 was sufficient for detection of VAChT Transporter in rat brain using immunohistochemistry analysis and goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 56kDa.
This antibody was formerly sold as S6-38.
靶标信息
This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: MGC12716; solute carrier family 18 (vesicular acetylcholine transporter), member 3; solute carrier family 18 (vesicular acetylcholine), member 3; solute carrier family 18 (vesicular monoamine) member 3; Solute carrier family 18 member 3; solute carrier family 18, member 3; VAChT; Vesicular acetylcholine transporter
基因别名: rVAT; SLC18A3; VACHT; VAT
UniProt ID: (Human) B2R7S1, (Rat) Q62666
Entrez Gene ID: (Human) 6572, (Mouse) 20508, (Rat) 60422
