Invitrogen

VAMP2 Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

2个已发表图片

9 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

图: 1 / 4

VAMP2 Antibody (PA1-766) in WB

Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Lung (Lane 3), Rat Lung (Lane 4), Rat Liver (Land 5) and Rat Kidney (Lane 6). The blot was probed with Anti- VAMP2 Rabbit Polyclonal Antibody (Product # PA1-766, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to VAMP2 was observed in Mouse Brain, Rat Brain and no... View More

VAMP2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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VAMP2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue models owing to their inherent genetic constitution. Relative expression of VAMP2 was observed in Mouse Brain, Rat Brain, Mouse Lung, Rat Lung, Rat Liver and Rat Kidney in Western Blot using VAMP2 Polyclonal Antibody (Product # PA1-766). VAMP2 is reported to be expressed in brain tissue and not in other tissues like lung, liver and kidney. {RE}

产品信息

PA1-766

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

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免疫细胞化学 (ICC/IF)

Assay-dependent

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免疫沉淀 (IP)

Assay-dependent

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其他PubMed (Misc)

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产品规格

种属反应

Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues M(1) S A T A A T V P P A A P A G E G G(18) C of rat VAMP-2.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2304164

产品详细信息

PA1-766 detects vesicle associated membrane protein 2 (VAMP-2) from rat and mouse tissues.

PA1-766 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody detects a 19 kDa protein representing VAMP-2 from rat brain whole protein extract.

The PA1-766 immunogen is a synthetic peptide corresponding to residues M(1) S A T A A T V P P A A P A G E G G(18) C of rat VAMP-2. This immunizing peptide (Cat. # PEP-101) is available for use in neutralization and control experiments.

靶标信息

The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer's disease, VAMP levels of all isoforms appear to be significantly lowered. It is suggested that VAMP-2 is a resident protein of the insulin-sensitive glucose transporter type 4 (GLUT4) compartment and that it is required for GLUT4 vesicle incorporation into the cell surface in response to insulin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Interaction of Munc18c and syntaxin4 facilitates invadopodium formation and extracellular matrix invasion of tumor cells.

The Journal of biological chemistry

Brasher MI,Martynowicz DM,Grafinger OR,Hucik A,Shanks-Skinner E,Uniacke J,Coppolino MG

Published figure using VAMP2 polyclonal antibody (Product # PA1-766) in Immunoprecipitation

Fri Sep 29 00:00:00 EDT 2017

Interaction of Munc18c and syntaxin4 facilitates invadopodium formation and extracellular matrix invasion of tumor cells.

The Journal of biological chemistry

Brasher MI,Martynowicz DM,Grafinger OR,Hucik A,Shanks-Skinner E,Uniacke J,Coppolino MG

Published figure using VAMP2 polyclonal antibody (Product # PA1-766) in Immunoprecipitation

Fri Sep 29 00:00:00 EDT 2017

Cellular compartmentation of energy metabolism: creatine kinase microcompartments and recruitment of B-type creatine kinase to specific subcellular sites.

Amino acids

Schlattner U,Klaus A,Ramirez Rios S,Guzun R,Kay L,Tokarska-Schlattner M

PA1-766 was used in western blot to study creatine kinase microcompartments and energy metabolism that recruits B-type creatine kinase to specific subcellular sites

Mon Aug 01 00:00:00 EDT 2016

Compensatory Islet Response to Insulin Resistance Revealed by Quantitative Proteomics.

Journal of proteome research

El Ouaamari A,Zhou JY,Liew CW,Shirakawa J,Dirice E,Gedeon N,Kahraman S,De Jesus DF,Bhatt S,Kim JS,Clauss TR,Camp DG,Smith RD,Qian WJ,Kulkarni RN

PA1-766 was used in western blot to identify alterations in the islet proteome that characterize the adaptive response

Fri Aug 07 00:00:00 EDT 2015

The Rab-GTPase-activating protein TBC1D1 regulates skeletal muscle glucose metabolism.

American journal of physiology. Endocrinology and metabolism

Szekeres F,Chadt A,Tom RZ,Deshmukh AS,Chibalin AV,Björnholm M,Al-Hasani H,Zierath JR

PA1-766 was used in western blot to study the role of the Rab-GTPase-activating protein TBC1D1 in regulating skeletal muscle glucose metabolism

Wed Aug 15 00:00:00 EDT 2012

American journal of physiology. Endocrinology and metabolism

Niu W,Bilan PJ,Ishikura S,Schertzer JD,Contreras-Ferrat A,Fu Z,Liu J,Boguslavsky S,Foley KP,Liu Z,Li J,Chu G,Panakkezhum T,Lopaschuk GD,Lavandero S,Yao Z,Klip A

PA1-766 was used in western blot to develop a cellular model for glucose uptake following muscle contraction

Sat May 01 00:00:00 EDT 2010

Cleavage of SNAP-25 and VAMP-2 impairs store-operated Ca2+ entry in mouse pancreatic acinar cells.

American journal of physiology. Cell physiology

Rosado JA,Redondo PC,Salido GM,Sage SO,Pariente JA

PA1-766 was used in western blot to demonstrate the role of SNAP-25 and VAMP-2 in the activation of SOCE in pancreatic acinar cells

Sat Jan 01 00:00:00 EST 2005

Evidence that an isoform of calpain-10 is a regulator of exocytosis in pancreatic beta-cells.

Molecular endocrinology (Baltimore, Md.)

Marshall C,Hitman GA,Partridge CJ,Clark A,Ma H,Shearer TR,Turner MD

PA1-766 was used in western blot to study the function ofa calpain-10 isoform as an exocytosis trigger in pancreatic beta-cells.

Sat Jan 01 00:00:00 EST 2005

The septin CDCrel-1 is dispensable for normal development and neurotransmitter release.

Molecular and cellular biology

Peng XR,Jia Z,Zhang Y,Ware J,Trimble WS

PA1-766 was used in immunofluorescence to probe VAMP-2 in primary cultures of mouse hippocampal neurons.

Tue Jan 01 00:00:00 EST 2002

生物信息学

蛋白别名: Synaptobrevin 2 (vesicle-associated membrane protein VAMP-2); synaptobrevin II; Synaptobrevin-2; vamp; VAMP-2; vesicle-associated membrane 2; Vesicle-associated membrane protein (synaptobrevin 2); Vesicle-associated membrane protein 2

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基因别名: RATVAMPB; RATVAMPIR; SYB; Syb-2; Syb2; sybII; Vamp2

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UniProt ID: (Mouse) P63044, (Rat) P63045

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Entrez Gene ID: (Mouse) 22318, (Rat) 24803

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功能