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VAMP2 Antibody (PA5-85779) in ICC/IF
VAMP2 Polyclonal Antibody detects VAMP2 protein at synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: VAMP2 protein stained by VAMP2 Polyclonal Antibody (Product # PA5-85779) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500. Blue:Fluoroshield with DAPI .
产品信息
PA5-85779
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse VAMP2.
偶联物
形式
Liquid
浓度
0.38 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 1% BSA, 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2792915
产品详细信息
Keep as concentrated solution.
Predicted reactivity: Mouse (100%), Rat (100%), Rhesus Monkey (100%), Bovine (100%).
Positive Control: mouse brain, rat brain.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer's disease, VAMP levels of all isoforms appear to be significantly lowered. It is suggested that VAMP-2 is a resident protein of the insulin-sensitive glucose transporter type 4 (GLUT4) compartment and that it is required for GLUT4 vesicle incorporation into the cell surface in response to insulin.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Synaptobrevin 2 (vesicle-associated membrane protein VAMP-2); synaptobrevin II; Synaptobrevin-2; vamp; VAMP-2; vesicle-associated membrane 2; Vesicle-associated membrane protein (synaptobrevin 2); Vesicle-associated membrane protein 2
基因别名: RATVAMPB; RATVAMPIR; SYB; Syb-2; Syb2; sybII; Vamp2
UniProt ID: (Mouse) P63044, (Rat) P63045
Entrez Gene ID: (Mouse) 22318, (Rat) 24803
protein complex assembly
vesicle fusion
response to glucose
protein transport
synaptic vesicle exocytosis
vesicle-mediated transport
calcium ion regulated exocytosis
regulation of exocytosis
regulation of calcium ion-dependent exocytosis
cellular response to insulin stimulus
Golgi to plasma membrane protein transport
eosinophil degranulation
long-term synaptic potentiation
regulation of vesicle-mediated transport
membrane fusion
positive regulation of intracellular protein transport
regulation of delayed rectifier potassium channel activity
neurotransmitter secretion
neutrophil degranulation
natural killer cell degranulation
mucus secretion
regulation of histamine secretion by mast cell
