Invitrogen

VEGF-165 Monoclonal Antibody, PeproTech®

3 篇参考文献
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产品信息

500-M88-500UG

应用
建议稀释比
已发表文章

免疫印迹 (WB)

0.25-0.50 µg/mL
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酶联免疫吸附实验 (ELISA)

2.0-4.0 µg/mL
-

其他PubMed (Misc)

-
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产品规格

种属反应

Human

已发表种属

Human, Rat

宿主/亚型

Mouse

分类

Monoclonal

类型

Antibody

抗原

E.coli-derived Recombinant Human VEGF 165
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

纯化类型

Protein A

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

RRID

AB_3074171

产品详细信息

AA Sequence of recombinant protein: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.

Preparation: Produced in BALB/c mice immunized with highly pure Recombinant Human VEGF 165. Anti-Human VEGF 165-specific antibody was purified from cell culture by Protein A affinity chromatography.

Sandwich ELISA: Assuming 100 µL/well, a concentration of 2.0-4.0 µg/mL of this antibody will detect at least 100 pg/mL of Recombinant Human VEGF 165 when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human VEGF 165 (500-P10BT) as the detection antibody at a concentration of approximately 0.25- 1.0 µg/mL.

Western Blot: To detect Human VEGF 165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human VEGF 165 is 0.25-0.50 ng/lane, under non-reducing conditions.

靶标信息

The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: H-VEGF-165; L-VEGF; M-VEGF-165; Vascular endothelial growth factor A, long form; vascular endothelial growth factor A121; vascular endothelial growth factor A165; Vascular permeability factor; VEGF165; VPF

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基因别名: MVCD1; VEGF; VEGFA; VPF

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UniProt ID: (Human) H3BLW7

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Entrez Gene ID: (Human) 7422

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功能
fibronectin binding cytokine activity platelet-derived growth factor receptor binding vascular endothelial growth factor receptor binding protein binding growth factor activity heparin binding chemoattractant activity identical protein binding protein homodimerization activity vascular endothelial growth factor receptor 1 binding vascular endothelial growth factor receptor 2 binding protein heterodimerization activity receptor agonist activity extracellular matrix binding growth factor
参与通路
negative regulation of transcription from RNA polymerase II promoter angiogenesis ovarian follicle development patterning of blood vessels vasculogenesis response to hypoxia in utero embryonic development kidney development positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation cell migration involved in sprouting angiogenesis positive regulation of neuroblast proliferation positive regulation of mesenchymal cell proliferation positive regulation of receptor internalization platelet degranulation positive regulation of leukocyte migration heart morphogenesis outflow tract morphogenesis coronary vein morphogenesis nervous system development mesoderm development lactation positive regulation of cell proliferation regulation of cell shape positive regulation of endothelial cell migration positive regulation of gene expression monocyte differentiation macrophage differentiation lung development positive regulation of cell migration regulation of cGMP metabolic process epithelial cell differentiation positive regulation of vascular endothelial growth factor receptor signaling pathway post-embryonic camera-type eye development positive regulation of protein complex assembly positive regulation of protein autophosphorylation activation of protein kinase activity positive regulation of CREB transcription factor activity positive regulation of peptidyl-serine phosphorylation tube formation endothelial cell chemotaxis cellular response to vascular endothelial growth factor stimulus lymph vessel morphogenesis positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway VEGF-activated neuropilin signaling pathway growth eye photoreceptor cell development positive regulation of tyrosine phosphorylation of Stat3 protein negative regulation of apoptotic process surfactant homeostasis negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAP kinase activity positive regulation of blood vessel endothelial cell migration positive regulation of angiogenesis positive regulation of cell adhesion positive regulation of transcription from RNA polymerase II promoter vascular endothelial growth factor receptor signaling pathway cell maturation camera-type eye morphogenesis cardiac muscle fiber development branching morphogenesis of an epithelial tube positive regulation of axon extension involved in axon guidance artery morphogenesis positive regulation of epithelial cell proliferation positive regulation of peptidyl-tyrosine phosphorylation positive chemotaxis positive regulation of positive chemotaxis induction of positive chemotaxis positive regulation of cellular component movement positive regulation of cell division positive regulation of focal adhesion assembly primitive erythrocyte differentiation mammary gland alveolus development cardiac vascular smooth muscle cell development coronary artery morphogenesis regulation of transcription from RNA polymerase II promoter in response to hypoxia positive regulation of transcription from RNA polymerase II promoter in response to hypoxia cellular response to hypoxia dopaminergic neuron differentiation commissural neuron axon guidance positive regulation of protein kinase C signaling positive regulation of cell migration involved in sprouting angiogenesis positive regulation of branching involved in ureteric bud morphogenesis regulation of retinal ganglion cell axon guidance positive regulation of peptidyl-tyrosine autophosphorylation positive regulation of p38MAPK cascade positive regulation of histone deacetylase activity positive regulation of retinal ganglion cell axon guidance positive regulation of protein kinase D signaling
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