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A 1:500 dilution of MA5-45924 was sufficient for detection of VGAT in 15 ug of Rat Brain Membrane by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects 57 kDa.
The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles (McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).
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